DEVELOPMENTALLY EXPRESSED O-ACETYL GANGLIOSIDE GT3 IN FETAL-RAT CEREBRAL-CORTEX
DEVELOPMENTALLY EXPRESSED O-ACETYL GANGLIOSIDE GT3 IN FETAL-RAT CEREBRAL-CORTEX
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DOI：
10.1016/0304-3940(89)90225-5
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发表时间：
1989-11-20
影响因子：
2.5
通讯作者：
ANDO, S
中科院分区：
文献类型：
--
作者：
HIRABAYASHI, Y;HIROTA, M;ANDO, S
针对鸡神经管建立的单抗M6704被证明识别三唾液酸基残基NeuAc.alpha.2-8NeuAc.Alpha。2-8NeuAc.α2-3-R的C系列神经节苷脂。用该抗体检测了胎鼠大脑皮层C系列神经节苷脂的发育变化。双向薄层层析(TLC)酶免疫染色分析表明，碱处理使GT3含量显著增加，占GT3总量的85%以上。在C流感病毒受体破坏酶-唾液酸O-乙酰酯酶的作用下，碱不稳定形式很容易降解为GT3，表明该抗原很可能是含有GT3的9-O-乙酰基-NeuAc。神经节苷脂在妊娠第14天开始高度浓缩，以后逐渐减少，成年大鼠大脑皮质中未检测到神经节苷脂。
Monoclonal antibody M6704, established against the chick neural tube, was shown to recognize a trisialosyl residue, NeuAc.alpha.2-8 NeuAc.alpha. 2-8NeuAc.alpha.2-3-R of C-series gangliosides. Using this antibody, the developmental changes of C-series gangliosides in fetal rat cerebral cortex have been examined. Two dimensional thin layer chromatography (TLC) enzyme-immunostaining analysis revealed that alkali treatment resulted in a great increase in GT3 that amounted to more than 85% of the total GT3 detected. The alkali-labile form was easily degraded to form GT3 by the action of the receptor-destroying enyzme of influenza C virus, sialate O-acetylesterase, indicating that the antigen was most probably 9-O-acetyl-NeuAc containing GT3. The ganglioside was highly enriched at the 14th gestation day, gradually decreased, and was not detected in adult rat cerebral cortex.