Fusion between disk membranes and plasma membrane of bovine photoreceptor cells is calcium dependent.
Fusion between disk membranes and plasma membrane of bovine photoreceptor cells is calcium dependent.
复制标题
牛感光细胞的盘膜和质膜之间的融合是钙依赖性的。
DOI：
10.1021/bi00130a002
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Yeagle,PL
中科院分区：
文献类型：
--
作者：
Boesze-Battaglia,K;Albert,AD;Yeagle,PL
材料和方法制备ROS膜。ROS盘和质膜均由同一棒状外段制备而成。简单地说，ROS是从冷冻的牛视网膜(Lawson；林肯，内布拉斯加州)中分离出来的，正如Papermaster和Dreyer(1974)所描述的那样。从相同的蔗糖密度梯度中分离出盘膜和质膜。后者按照Molday和Molday(1987)的描述制备，并由Boesze-Battaglia和Albert(1989)修改。质膜从蓖麻毒素琼脂糖凝胶(密苏里州圣路易斯西格玛)上洗脱
Materials and MethodsPreparation ROS Membranes. Both ROS disk and plasma membranes were prepared as descirbed from the same rod outer segment preparation. Briefly, ROS were isolated from frozen bovine retinas (Lawson; Lincoln, Nebraska) as described by Papermaster and Dreyer (1974). The disk mem-branes and plasma membranes were isolated from the same sucrose density gradient. The latter was prepared as described by Molday and Molday (1987) and modified by Boesze-Battaglia and Albert (1989). The plasma membrane was eluted from the ricin-agarose (Sigma, St. Louis, MO) with