Endocrine disruptive effects of chemicals eluted from nitrile-butadiene rubber gloves using reporter gene assay systems.
Endocrine disruptive effects of chemicals eluted from nitrile-butadiene rubber gloves using reporter gene assay systems.
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使用报告基因检测系统从丁腈橡胶手套中洗脱的化学物质的内分泌干扰作用。
DOI：
10.1248/bpb.31.375
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发表时间：
2008
影响因子：
2
通讯作者：
M. Iida
中科院分区：
文献类型：
--
作者：
K. Satoh;Ryouichi Nonaka;K. Ohyama;F. Nagai;A. Ogata;M. Iida
使用丁腈橡胶（NBR）制成的一次性手套而不是含有邻苯二甲酸二（2-乙基己基）酯（DEHP）的聚氯乙烯手套接触食品，因为怀疑邻苯二甲酸二酯（包括DEHP）具有内分泌破坏作用。然而，4，4 '-亚丁基双（6-叔丁基间甲酚）（BBBC）、2，4-二叔丁基苯酚和2，2，4-三甲基-1，3-戊二醇二异丁酸酯可从NBR手套中洗脱，也可能在食品中检测到。在这项研究中，我们分别使用表达雄激素受体（AR）（AR-EcoScreen系统）和雌激素受体（ER）（MVLN细胞）的稳定转染报告基因细胞系，通过雄激素受体（AR）和雌激素受体（ER）介导的途径检查了这些化学物质的内分泌干扰作用。我们还研究了这些化学物质与AR和ER的结合活性。BBBC对拮抗雄激素的IC_（50）值在10（-6）M范围内。其抑制强度约为已知雄激素拮抗剂1，1 '-（2，2-二氯亚乙基）双[4-氯苯]（p，p'-DDE）的5倍，与双酚A相似。BBBC对拮抗雌激素的IC_（50）值在10 ~（-6）M范围内。这些结果表明，BBBC及其结构同源物4，4 '-硫代双（6-叔丁基间甲酚）是雄激素和雌激素拮抗剂。因此，有必要在体内研究这些化学物质，并阐明其对内分泌系统的影响。
Disposable gloves made of nitrile-butadiene rubber (NBR) are used for contact with foodstuffs rather than polyvinyl chloride gloves containing di(2-ethylhexyl)phthalate (DEHP), because endocrine-disruptive effects are suspected for phthalate diesters including DEHP. However, 4,4'-butylidenebis(6-t-butyl-m-cresol) (BBBC), 2,4-di-t-butylphenol, and 2,2,4-trimetyl-1,3-pentanediol diisobutyrate can be eluted from NBR gloves, and possibly also detected in food. In this study, we examined the endocrine-disrupting effects of these chemicals via androgen receptor (AR) and estrogen receptor (ER)-mediated pathways using stably transfected reporter gene cell lines expressing AR (AR-EcoScreen system) and ER (MVLN cells), respectively. We also examined the binding activities of these chemicals to AR and ER. The IC50 value of BBBC for antagonistic androgen was in the range of 10(-6)M. The strength of inhibition was about 5 times that of a known androgen antagonist, 1,1'-(2,2-dichloroethylidene)bis[4-chlorobenzene] (p,p'-DDE), and similar to that of bisphenol A. The IC50 value of BBBC for antagonistic estrogen was in the range of 10(-6)M. These results suggest that BBBC and its structural homologue, 4,4'-thiobis(6-t-butyl-m-cresol) are androgen and estrogen antagonists. It is therefore necessary to study these chemicals in vivo, and clarify their effect on the endocrine system.
宏观剪切场应用下致密胶体悬浮液的微观流变学
DOI：
--
发表时间：
2021
期刊：
影响因子：
--
作者：
吉良和真;荊原佳祐;水野大介
通讯作者：
水野大介