Myosin functions in Xenopus retinal ganglion cell growth cone motility in vivo
Myosin functions in Xenopus retinal ganglion cell growth cone motility in vivo
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DOI：
10.1002/(sici)1097-4695(19970605)32:6
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发表时间：
1997-06-05
期刊：
影响因子：
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通讯作者：
Harris, WA
中科院分区：
文献类型：
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作者：
Ruchhoeft, ML;Harris, WA
肌球蛋白的作用在非洲爪蟾视网膜神经节细胞生长锥运动的视束进行了研究，使用两种不同的特异性药理学抑制剂。2，3-丁二酮单肟（BDM）破坏所有肌球蛋白的肌球蛋白-肌动蛋白相互作用，ML-7特异性抑制肌球蛋白II的活化。这两种抑制剂均导致生长锥呈现塌陷形态和生长锥速度降低。有趣的是，两种抑制剂的作用虽然相似，但明显可区分，这提高了不同肌球蛋白在生长锥运动中可能具有不同功能作用的可能性，BDM引起生长锥撤回板状伪足和一些丝状伪足并最终冻结，而ML-7引起完全塌陷和缩回，当单独应用时没有作用的BDM和ML-7浓度在同时应用时停止生长锥，表明这些抑制剂协同作用于肌球蛋白功能，从而提供特异性的证据，这些结果意味着，正常的生长锥运动的分子和空间复杂的环境中的活的大脑需要肌球蛋白的功能。(C)John Wiley & Sons，Inc.
The role of myosins in Xenopus retinal ganglion cell growth cone motility in the optic tract was studied using two pharmacologic inhibitors with different specificities. 2,3-Butanedione monoxime (BDM) disrupts myosin-actin interactions of all myosins, and ML-7 specifically inhibits activation of myosin II, Both inhibitors caused growth cones to assume a collapsed morphology and decreased growth cone speed, Similar effects were observed in vitro. Interestingly, the effects of the two inhibitors, while similar, were clearly distinguishable, raising the possibility that different myosins may have different functional roles in growth cone motility, BDM caused growth cones to withdraw lamellipodia and some filopodia and eventually to freeze, whereas ML-7 caused total collapse and retraction, Concentrations of BDM and ML-7 that had no effect when applied independently stopped growth cones when applied simultaneously, suggesting that these inhibitors act synergistically on myosin function, thus providing evidence of specificity, These results imply that normal growth cone motility in the molecularly and spatially complex environment of the living brain requires myosin function. (C) 1997 John Wiley & Sons, Inc.