Photo-induced proton gradients and ATP biosynthesis produced by vesicles encapsulated in a silica matrix
Photo-induced proton gradients and ATP biosynthesis produced by vesicles encapsulated in a silica matrix
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DOI：
10.1038/nmat1322
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发表时间：
2005-03-01
期刊：
影响因子：
41.2
通讯作者：
Montemagno, C
中科院分区：
文献类型：
--
作者：
Luo, TJM;Soong, R;Montemagno, C
溶胶-凝胶固定化可溶性蛋白质已被证明是一种可行的方法，用于稳定透明无机基质中的各种蛋白质。膜结合蛋白的包封受到的关注要少得多，尽管这一领域的工作表明了微阵列技术和高通量药物筛选的潜在机会。本论文描述了一种脂质体/溶胶-凝胶体系结构，其中脂质体提供膜结构和蛋白质取向的两个跨膜蛋白，细菌视紫红质（bR）和F0 F1-ATP合酶;溶胶-凝胶包封将脂质体溶液转化为一个强大的材料，而不损害掺入的蛋白质的内在活性。在这里，我们报告两种不同的蛋白脂质体掺杂的凝胶（蛋白凝胶），其性质是由跨膜蛋白。含有bR蛋白脂质体的蛋白凝胶在用可见光照射时表现出稳定的质子梯度，而含有具有bR和F0 F1-ATP合酶两者的蛋白脂质体的蛋白凝胶将光诱导的质子梯度与ATP的产生偶联。这些结果表明，基于脂质体/溶胶-凝胶体系结构的材料能够利用跨膜蛋白的性质，并实现各种应用，从发电和储能到为分子马达供电，并代表了一种用于在固态基质中进行复杂化学合成的新技术。
Sol–gel immobilization of soluble proteins has proven to be a viable method for stabilizing a wide variety of proteins in transparent inorganic matrices,,. The encapsulation of membrane-bound proteins has received much less attention, although work in this area suggests potential opportunities in microarray technology and high-throughput drug screening,. The present paper describes a liposome/sol–gel architecture in which the liposome provides membrane structure and protein orientation to two transmembrane proteins, bacteriorhodopsin (bR) and F0F1-ATP synthase; the sol–gel encapsulation converts the liposomal solution into a robust material without compromising the intrinsic activity of the incorporated proteins. Here we report on two different proteoliposome-doped gels (proteogels) whose properties are determined by the transmembrane proteins. Proteogels containing bR proteoliposomes exhibit a stable proton gradient when irradiated with visible light, whereas proteogels containing proteoliposomes with both bR and F0F1-ATP synthase couple the photo-induced proton gradient to the production of ATP. These results demonstrate that materials based on the liposome/sol–gel architecture are able to harness the properties of transmembrane proteins and enable a variety of applications, from power generation and energy storage to the powering of molecular motors, and represent a new technology for performing complex chemical synthesis in a solid-state matrix.