Expression and Interactions Between Cell Adhesion Molecules CD44v6 and E-Cadherin in Human Gliomas
Expression and Interactions Between Cell Adhesion Molecules CD44v6 and E-Cadherin in Human Gliomas
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DOI：
10.17219/acem/37261
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发表时间：
2014-09-01
影响因子：
2.1
通讯作者：
Paradowski, Boguslaw
中科院分区：
文献类型：
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作者：
Bar, Julia K.;Zub, Leslaw;Paradowski, Boguslaw
背景神经胶质瘤是一组异质性肿瘤，具有相同的组织学特征，但行为不同。神经胶质瘤的特征是生物学侵袭性和向周围健康脑组织的广泛浸润性生长。目的。在这项研究中，我们估计CD 44 v6和E-cadherin的表达和CD 44 v6和E-cadherin之间的相关性与胶质瘤恶性度。我们还分析了同一肿瘤样品中CD 44 v6和E-钙粘蛋白的同时表达，以确定生物学肿瘤行为。采用免疫组化方法检测92例福尔马林固定石蜡包埋胶质瘤组织中CD 44 v6和E-cadherin的表达。71.6%的胶质瘤表达CD 44 v6。低（I级）与高（IV级）以及I级与II级恶性胶质瘤中CD 44 v6表达的阳性病例频率之间存在统计学显著差异（p = 0.001）。28.8%的胶质瘤可见E-钙粘蛋白膜染色。E-cadherin在胶质瘤中的表达与胶质瘤分级无显著性差异（p > 0.05）。然而，在II级胶质瘤中发现E-钙粘蛋白的再表达。本组病例E-cadherin表达率为43.3%。为了确定CD 44 v6和E-cadherin表达之间的关系，我们分析了整个组中同一胶质瘤样本中CD 44 v6和E-cadherin的同时表达以及胶质瘤恶性程度。在所分析的胶质瘤中观察到所研究的生物标志物之间的正相关性（p = 0.004），但是CD 44 v6和E-cadherin的同时表达揭示了在胶质瘤恶性方面没有显著差异。本研究结果表明，E-cadherin水平可能反映胶质瘤的不同生物学特性，而CD 44 v6与肿瘤细胞恶性程度有关。在一组低级别胶质瘤中同时存在CD 44 v6和E-cadherin表明这两种分子可能会加强细胞迁移，并可能是胶质瘤侵袭性生长的标志。
Background. Gliomas are a heterogenous group of tumors that show the same histological features but differ in their behavior. Gliomas are characterized by biological aggressiveness and extensive infiltrative growth into surrounding healthy brain tissue.Objectives. In this study we estimated CD44v6 and E-cadherin expression and correlation between CD44v6 and E-cadherin in relation to glioma malignancy. We also analyzed simultaneous expression of CD44v6 and E-cadherin in the same tumor sample in order to determine the biological tumor behavior.Material and Methods. Expression of CD44v6 and E-cadherin was evaluated on ninety-two formalin-fixed paraffin-embedded glioma tissue blocks using immunohistochemistry (IHC).Results. CD44v6 expression was found in 71.6% of gliomas. There was a statistically significant difference between the frequency of positive cases for CD44v6 expression in low (grade I) vs. high (grade IV) as well as in grade I vs. grade II of glioma malignancy (p = 0.001). E-cadherin membrane staining was observed in 28.8% of gliomas. No significant differences were observed between E-cadherin expression and grade of gliomas (p > 0.05). However, re-expression of E-cadherin was found in grade II gliomas. In this group, E-cadherin expression was revealed in 43.3% of the cases. In order to define the relationship between CD44v6 expression and E-cadherin, we analyzed the simultaneous expression of CD44v6 and E-cadherin in the same glioma sample in the whole group and in respect to the degree of glioma malignancy. A positive correlation between studied biomarkers was observed in the analyzed gliomas (p = 0.004) but a simultaneous expression of CD44v6 and E-cadherin revealed no significant differences in respect to glioma malignancy.Conclusions. Our results showed that the level of E-cadherin might reflect different biological features of gliomas, whereas CD44v6 is associated with tumor cell malignancy. The simultaneous presence of CD44v6 and E-cadherin in a set of low-grade gliomas indicates that both these molecules might strengthen cell migration and may be a hallmark of glioma invasive growth.