Glucose-6-phosphate isomerase is not a specific autoantigen in rheumatoid arthritis
Glucose-6-phosphate isomerase is not a specific autoantigen in rheumatoid arthritis
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DOI：
10.1093/rheumatology/keg271
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发表时间：
2003-08-01
期刊：
影响因子：
5.5
通讯作者：
Venables, PJ
中科院分区：
文献类型：
--
作者：
Herve, CA;Wait, R;Venables, PJ
目标。为了验证葡萄糖-6-磷酸异构酶（GPI）是一种新的ra自身抗原的假设。采用市售的部分纯化兔GPI作为抗原，对23例类风湿性关节炎（RA）患者、25例干燥综合征患者、20例系统性红斑狼疮患者和20例健康对照者的88份血清样本进行酶联免疫吸附试验（ELISA）检测。在每个含有抗原（10马克杯/毫升）的重复孔旁边，包括未包被的阻塞重复孔（仅磷酸盐缓冲盐水）作为对照，用于每种血清的非特异性结合。我们还通过免疫印迹检测了73例血清中GPI制剂中与各种多肽结合的抗体。通过ELISA检测，RA患者和其他风湿病患者的血清结合水平较低，与正常对照无差异。免疫印迹法发现，70-80%的实验组均存在GPI多肽结合抗体。此外，我们还发现制备中还存在另一种被鉴定为磷酸葡萄糖糖化酶的多肽，并与人免疫球蛋白发生反应。我们的研究结果表明GPI不是RA的特异性自身抗原。
Objective. To test the hypothesis that glucose-6-phosphate isomerase (GPI) is a novel autoantigen in RA.Methods. Eighty-eight serum samples from 23 patients with rheumatoid arthritis (RA), 25 with Sjogren's syndrome, 20 with systemic lupus erythematosus and 20 healthy controls were tested by enzyme-linked immunosorbent assay (ELISA) using a commercially available, partially purified rabbit GPI as antigen. Beside each duplicate well containing antigen (10 mug/ml), uncoated blocked duplicate wells (phosphate-buffered saline only) were included as controls for non-specific binding for every serum tested. We also examined antibodies binding to various polypeptides in the GPI preparation by immunoblotting in 73 of the sera.Results. By ELISA, binding levels were low and there was no difference between serum from patients with RA, other rheumatic diseases and normal controls. By immunoblotting, antibodies binding to the GPI polypeptide were present in 70-80% of all groups tested. In addition, we showed that another polypeptide identified as phosphoglucomutase was also present in the preparation and reacted with human immunoglobulins.Conclusion. Our findings suggest that GPI is not a specific autoantigen in RA.