Differential nicotinic acetylcholine receptor subunit expression in the human hippocampus
Differential nicotinic acetylcholine receptor subunit expression in the human hippocampus
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DOI：
10.1016/s0891-0618(02)00100-x
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发表时间：
2003-02-01
影响因子：
2.8
通讯作者：
Court, JA
中科院分区：
文献类型：
--
作者：
Graham, AJ;Ray, MA;Court, JA
神经元烟碱乙酰胆碱受体（nAChR）是由α和β亚基组成的配体门控离子通道，具有特定的结构、功能和药理学特性。在这项研究中，使用免疫组织化学研究了α 3、α 4、α 7、β 2和β 4 nAChR亚基在人海马中的分布。大多数锥体神经元，前α细胞的内嗅皮质和齿状颗粒细胞的免疫反应的所有亚基。α 7免疫反应阳性细胞的小岛存在于外前下托。α 4和β 2以及α 3、α 4和β 2免疫反应纤维束分别存在于放射层和下托中，这表明nAChR可能在调节通过Schaffer侧支和沿着穿孔通路向海马的输入中发挥作用。一些星形胶质细胞对α 3、α 7和β 4亚基呈免疫反应性。所有亚基的免疫反应性与血管相关。这些结果表明，参与多种nAChR亚型在人类海马神经元和非神经元功能的调制。(C)2002 Elsevier Science B.V.保留所有权利。
Neuronal nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels composed of alpha and beta subunits with specific structural, functional and pharmacological properties. In this study the distribution of alpha3, alpha4, alpha7, beta2 and beta4 nAChR subunits in the human hippocampus was investigated using immunohistochemistry. Most pyramidal neurons, pre-alpha cells of the entorhinal cortex and dentate granule cells were immunoreactive for all subunits. Small islands of alpha7 immunoreactive cells were present in the outer presubiculum. alpha4 and beta2, and alpha3, alpha4 and beta2 immunoreactive fibre tracts were present in the stratum radiatum and subiculum, respectively, suggesting nAChRs may play a role in modulating inputs to the hippocampus via Schaffer collaterals and along the perforant pathway. Some astrocytes were immunoreactive for alpha3, alpha7 and beta4 subunits. Immunoreactivity to all subunits was noted in association with blood vessels. These results indicate the involvement of multiple nAChR subtypes in the modulation of both neuronal and non-neuronal functions in the human hippocampus. (C) 2002 Elsevier Science B.V. All rights reserved.