The cyanobacterium Gloeobacter violaceus PCC 7421 uses bacterial‐type phytoene desaturase in carotenoid biosynthesis
The cyanobacterium Gloeobacter violaceus PCC 7421 uses bacterial‐type phytoene desaturase in carotenoid biosynthesis
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DOI：
10.1016/j.febslet.2005.02.066
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发表时间：
2005-04
期刊：
影响因子：
3.5
通讯作者：
T. Tsuchiya;S. Takaichi;N. Misawa;T. Maoka;H. Miyashita;M. Mimuro
中科院分区：
文献类型：
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作者：
T. Tsuchiya;S. Takaichi;N. Misawa;T. Maoka;H. Miyashita;M. Mimuro
研究了蓝细菌Gloeulum violaceus PCC 7421的类胡萝卜素组成及其生物合成途径。β-胡萝卜素和（2S，2′S）-β-2，2 ′-二（α-l-岩藻糖苷）和海胆烯酮分别是主要和次要类胡萝卜素。我们确定了两个独特的类胡萝卜素生物合成的基因，在体内功能互补实验。在Gloeetum中，细菌型八氢番茄红素去饱和酶（CrtI）而不是植物型去饱和酶（CrtP和CrtQ）产生番茄红素。这是利用细菌型八氢番茄红素去饱和酶的产氧光合生物的第一个证明。我们还发现，海胆酮的合成是由CrtW催化，而不是CrtO。这些发现表明，粘多糖保留了类胡萝卜素生物合成的祖先特性。
Carotenoid composition and its biosynthetic pathway in the cyanobacterium Gloeobacter violaceus PCC 7421 were investigated. β-Carotene and (2S,2′S)-oscillol 2,2′-di(α-l-fucoside), and echinenone were major and minor carotenoids, respectively. We identified two unique genes for carotenoid biosynthesis using in vivo functional complementation experiments. In Gloeobacter, a bacterial-type phytoene desaturase (CrtI), rather than plant-type desaturases (CrtP and CrtQ), produced lycopene. This is the first demonstration of an oxygenic photosynthetic organism utilizing bacterial-type phytoene desaturase. We also revealed that echinenone synthesis is catalyzed by CrtW rather than CrtO. These findings indicated that Gloeobacter retains ancestral properties of carotenoid biosynthesis.