Differential gene expression and alternative splicing of survivin following mouse sciatic nerve injury
Differential gene expression and alternative splicing of survivin following mouse sciatic nerve injury
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DOI：
10.1038/sc.2009.26
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发表时间：
2009-10-01
期刊：
影响因子：
2.2
通讯作者：
Tavallaei, M.
中科院分区：
文献类型：
--
作者：
Amiri, S.;Movahedin, M.;Tavallaei, M.
研究设计：在成年雄性海军医学研究所小鼠中使用轴突切断模型的体内研究。目的：凋亡抑制蛋白（inhibitor of the apoptosis，IAP）家族的独特成员Survivin在胚胎发育过程中表达，但在终末分化的细胞和组织中检测不到。由于生存素在细胞增殖和凋亡细胞死亡中的重要作用，以及治疗神经系统损伤的必要性，我们监测了生存素基因表达及其在损伤小鼠坐骨神经内不同时间点的选择性剪接变化。将成年雄性海军医学研究所小鼠的坐骨神经横断，并使用半定量RT-PCR和免疫组织化学检测存活素在所解剖的神经的远侧和近侧部分以及动物的脊髓内的相应节段中的表达。Survivin在未损伤的坐骨神经中无表达，但在坐骨神经损伤后，其在离断的坐骨神经近端和远端逐渐表达上调（P < 0.05）。Survivin 140是损伤后表达的主要变异体，同时伴有Survivin 40的低表达（P < 0.05）。结论：Survivin在损伤的神经和脊髓中存在差异表达和剪接。未来的工作操纵的表达和/或剪接的生存素可以破译的潜在影响的生存素变体的神经和/或脊髓损伤的再生。Spinal Cord（2009）47，739-744; doi：10.1038/sc.2009.26; 2009年3月31日在线发表
Study design: In vivo studies using an axotomy model in adult male Naval Medical Research Institute mice.Objective: Survivin, a unique member of the inhibitor of the apoptosis (IAP) protein family, is expressed during embryonal development, but is undetectable in terminally differentiated cells and tissues. Owing to the vital role of survivin in cellular proliferation and apoptotic cell death, and also to the necessity of treatment of the nervous system injuries, we have monitored survivin gene expression as well as its alternative splicing changes at different time points within injured mouse sciatic nerves.Setting: Department of Genetics, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran.Methods: The sciatic nerves of adult male Naval Medical Research Institute mice were transected and the expression of survivin was examined in the distal and proximal parts of the dissected nerves as well as in the corresponding segments within the spinal cord of the animals, using semi-quantitative RT-PCR and immunohistochemistry.Results: Survivin is not expressed in undamaged sciatic nerves, but, after sciatic nerve injury, it is gradually upregulated in proximal and distal parts of the dissected nerve (P < 0.05). Survivin140 is the main variant expressed after injury, accompanied by a low expression of survivin40 (P < 0.05). There was no expression of the survivin121 variant after injury.Conclusions: Survivin is differentially expressed and spliced in damaged nerve and spinal cord. Future works on the manipulation of expression and/or the splicing of survivin could decipher the potential effects of survivin variants on the regeneration of nerve and/or spinal cord injuries. Spinal Cord (2009) 47, 739-744; doi: 10.1038/sc.2009.26; published online 31 March 2009