Bile acids enhance invasiveness of Cryptosporidium spp. into cultured cells
Bile acids enhance invasiveness of Cryptosporidium spp. into cultured cells
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DOI：
10.1128/iai.00169-06
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发表时间：
2006-06-01
影响因子：
3.1
通讯作者：
Tzipori, Saul
中科院分区：
文献类型：
--
作者：
Feng, Hanping;Nie, Weijia;Tzipori, Saul
胆盐如牛磺胆酸钠（NaTC）通常用于诱导隐孢子虫卵囊脱囊。在这里，我们表明，NaTC显着增强了几个培养的细胞系的新鲜excysted微小隐孢子虫和隐孢子虫人的子孢子的入侵。各种纯化的牛胆汁盐或总胆汁也能增强C.小的进一步的研究表明，NaTC增加子孢子的蛋白分泌和滑行运动，这是成功入侵的关键过程。这些观察结果可能会导致培养细胞的隐孢子虫感染性的改善，并有助于未来的宿主-寄生虫相互作用的研究。
Bile salts such as sodium taurocholate (NaTC) are routinely used to induce the excystation of Cryptosporidium oocysts. Here we show that NaTC significantly enhanced the invasion of several cultured cell lines by freshly excysted Cryptosporidium parvum and Cryptosporidium hominis sporozoites. A variety of purified bile salts or total bile from bovine also enhanced the invasion of cultured cells by C. parvum. Further studies demonstrated that NaTC increased protein secretion and gliding motility of sporozoites, the key processes for successful invasion. These observations may lead to improved Cryptosporidium infectivity of cultured cells and help future studies on the host-parasite interaction.