Up-regulation of SGTB is associated with neuronal apoptosis after neuroinflammation induced by lipopolysaccharide
Up-regulation of SGTB is associated with neuronal apoptosis after neuroinflammation induced by lipopolysaccharide
复制标题
脂多糖诱导的神经炎症后 SGTB 上调与神经元凋亡相关
DOI：
10.1007/s10735-013-9517-4
复制
发表时间：
2013-10-01
影响因子：
3.2
通讯作者：
Wu, Qiyun
中科院分区：
文献类型：
--
作者：
Cao, Maohong;Xu, Wei;Wu, Qiyun
SGTB（富含谷氨酰胺的小三角肽重复序列（TPR），β）在蛋白质-蛋白质相互作用中起着关键作用。近年来，SGTB与热休克同源蛋白（Hsc 70）/热休克蛋白（Hsp 70）的相互作用引起了人们的广泛关注。本研究旨在阐明脂多糖（LPS）诱导的神经炎症大鼠模型中SGTB表达和分布的动态变化。结果发现，注射LPS后，SGTB在凋亡神经元中的表达显著增加。本实验结果提示SGTB的上调可能与H2 O2刺激后神经元凋亡有关。此外，通过siRNA沉默培养的PC 12（嗜铬细胞瘤）中的SGTB表明，SGTB是氧化应激诱导的神经元凋亡所必需的。我们对细胞信号通路的研究可能为中枢神经系统炎症中神经元凋亡的防治提供新的策略。
SGTB (Small glutamine-rich tetratricopeptide repeat (TPR)-containing, beta) plays a critical role in protein-protein interactions. The interaction between SGTB and heat shock cognate protein (Hsc70)/heat shock protein (Hsp70) has aroused much attention in recent years. The present study was designed to elucidate dynamic changes in SGTB expression and distribution in the cerebral cortex in a lipopolysaccharide (LPS)-induced neuroinflammation rat model. It was found that SGTB expression was increased significantly in apoptotic neurons after LPS injection. The result of our in vitro study suggested that SGTB up-regulation might be associated with neuronal apoptosis after H2O2 challenge. In addition, silencing of SGTB in cultured PC12 (Pheochromocytoma) by siRNA indicated that SGTB was required for neuronal apoptosis induced by oxidative stress. Our finding about the cellular signal pathway may provide a new strategy against neuronal apoptosis in neuroinflammation in CNS.