IMMUNOCHEMICAL DEMONSTRATION OF INCREASED ACCUMULATION OF TYROSINE-HYDROXYLASE PROTEIN IN SYMPATHETIC-GANGLIA AND ADRENAL-MEDULLA ELICITED BY RESERPINE
IMMUNOCHEMICAL DEMONSTRATION OF INCREASED ACCUMULATION OF TYROSINE-HYDROXYLASE PROTEIN IN SYMPATHETIC-GANGLIA AND ADRENAL-MEDULLA ELICITED BY RESERPINE
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DOI：
10.1073/pnas.70.10.2767
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发表时间：
1973-01-01
影响因子：
11.1
通讯作者：
REIS, D
中科院分区：
文献类型：
--
作者：
JOH, TH;GEGHMAN, C;REIS, D
大鼠长期服用利血平可增加交感神经节和肾上腺髓质中酪氨酸羟化酶（EC 1.14.3.x）的活性，该酶催化儿茶酚胺生物合成中的限速步骤。肾上腺和受神经支配的上级颈神经节中的酶的免疫化学滴定表明，增强的酶活性完全归因于更特异的酶蛋白的积累，而不是预先存在的酶分子的激活。
Chronic administration of reserpine to rats increases, in sympathetic ganglia and adrenal medulla, the activity of tyrosine hydroxylase (EC 1.14.3.x), the enzyme catalyzing the rate-limiting step in the biosynthesis of catecholamines. Immunochemical titration of the enzyme in both adrenal gland and innervated superior cervical ganglia demonstrates that enhanced enzyme activity is entirely attributable to accumulation of more specific enzyme protein and not activation of preexistent enzyme molecules.