Polytrodes: High-density silicon electrode arrays for large-scale multiunit recording
Polytrodes: High-density silicon electrode arrays for large-scale multiunit recording
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DOI：
10.1152/jn.01023.2004
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发表时间：
2005-05-01
影响因子：
2.5
通讯作者：
Swindale, NV
中科院分区：
文献类型：
--
作者：
Blanche, TJ;Spacek, MA;Swindale, NV
我们开发了各种54通道高密度硅电极阵列（多电极），旨在记录跨越大脑毫米的大量神经元。在猫的视觉皮层中，可以同时记录超过100个分离良好的神经元。使用标准的聚类方法，多边形提供了一个质量的单一单位的隔离，超过了四极。成功的体内记录和精确的电极定位的指南进行了说明。我们还描述了一个高带宽的连续数据采集系统，专门为多电极和自动阻抗计测试多电极网站的完整性。尽管具有比早期的这种类型的硅基电极更小的互连节距，但这些多电极具有可忽略的通道串扰、相当的可靠性和低的位点阻抗，并且能够以最小的组织损伤进行高保真多单元记录。平面电极阵列的相对良性性质在组织学上和实验中都是明显的，在实验中，多极在许多小时的时间内重复前进和缩回数百微米。它是可能的，以保持稳定的记录，从活跃的神经元相邻的多电极，而不改变其绝对位置，神经生理或感受野特性。
We developed a variety of 54-channel high-density silicon electrode arrays (polytrodes) designed to record from large numbers of neurons spanning millimeters of brain. In cat visual cortex, it was possible to make simultaneous recordings from > 100 well-isolated neurons. Using standard clustering methods, polytrodes provide a quality of single-unit isolation that surpasses that attainable with tetrodes. Guidelines for successful in vivo recording and precise electrode positioning are described. We also describe a high-bandwidth continuous data-acquisition system designed specifically for polytrodes and an automated impedance meter for testing polytrode site integrity. Despite having smaller interconnect pitches than earlier silicon-based electrodes of this type, these polytrodes have negligible channel crosstalk, comparable reliability, and low site impedances and are capable of making high-fidelity multiunit recordings with minimal tissue damage. The relatively benign nature of planar electrode arrays is evident both histologically and in experiments where the polytrode was repeatedly advanced and retracted hundreds of microns over periods of many hours. It was possible to maintain stable recordings from active neurons adjacent to the polytrode without change in their absolute positions, neurophysiological or receptive field properties.