MITOTIC ACTIVITY IN HEMOCYTES OF ONCOPELTUS FASCIATUS (DALL)

MITOTIC ACTIVITY IN HEMOCYTES OF ONCOPELTUS FASCIATUS (DALL)
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DOI:
10.1016/0014-4827(65)90067-4
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发表时间:
1965-01-01
影响因子:
3.7
通讯作者:
OCONNOR, GM
OCONNOR, GM
中科院分区:
医学3区
文献类型:
--
作者:
FEIR, D;OCONNOR, GM

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连续几天在整个第五体育场将氚化胸苷注射到单独的第五龄马利筋虫群中。在注射后每24小时从不同的昆虫制备血淋巴涂片,持续第五阶段的持续时间。测定氚化胸苷掺入的细胞百分比、有丝分裂中的细胞百分比和掺入的有丝分裂像百分比。无论在第5个时期注射胸苷的哪一天,血细胞中氚化胸苷掺入的高峰都出现在蜕皮后5-6天和9-10天。在整个体育场内观察到不同百分比(0-15)的有丝分裂。有丝分裂指数与昆虫的蜕皮后年龄,注射日,或氚化胸苷掺入的峰值没有相关性。
Tritiated thymidine was injected into separate groups of fifth instar milkweed bugs on successive days throughout the fifth stadium. Hemolymph smears were made from different insects every 24 hours after the injection for the duration of the fifth stadium. The percentage of cells with tritiated thymidine incorporation, the percentage of cells in mitosis, and the percentage of mitotic figures with incorporation was determined. The high peaks of tritiated thymidine incorporation into the hemocytes occurred on 5-6 days and 9-10 days after the molt regardless of the day in the fifth stadium when the injection of thymidine was given. Variable percentages (0-15) of mitosis were seen throughout the stadium. There was no correlation of the mitotic index with the post molt age of the insect, day of injection, or peaks of tritiated thymidine incorporation.