In vivo transfer of a foreign gene to keratinocytes using the hemagglutinating virus of Japan-liposome method.
In vivo transfer of a foreign gene to keratinocytes using the hemagglutinating virus of Japan-liposome method.
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使用日本血凝病毒-脂质体方法将外源基因体内转移至角质形成细胞。
DOI：
10.1111/1523-1747.ep12334229
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Yasufmi Kaneda
中科院分区：
文献类型：
--
作者：
D. Sawamura;Xianmim Meng;S. Ina;H. Ishikawa;K. Tamai;K. Nomura;K. Hanada;I. Hashimoto;Yasufmi Kaneda
日本血凝病毒(HVJ)-脂质体方法包括将DNA和核蛋白包裹在脂质体中，并使用HVJ促进脂质体与细胞膜的融合。这种方法已成功地用于体内基因转移到各种类型的组织。在这项研究中，我们研究了这种方法是否有效地将基因转移到体内正常和恶性转化的角质形成细胞。我们将含有β-半乳糖苷酶基因的HVJ-脂质体复合体(HLC)应用于无毛大鼠的脱毛皮肤，并检测了处理后皮肤角质形成细胞中的酶活性。将这种方法与最近被证明用于体内角质形成细胞基因转移的裸DNA注射方法进行了比较，发现后者的转移效率大约是前者的5倍。我们通过检测含有单纯疱疹病毒胸苷激酶基因的HLC对小鼠鳞状细胞癌生长的影响，评价了HVJ-脂质体方法将基因转移到转化的角质形成细胞的效果。小鼠瘤内局部注射HLC后给予更昔洛韦，肿瘤生长受到抑制。这些结果表明，该方法适用于角质形成细胞的体内基因转移，为角质形成细胞生物学的基础研究和未来的角质形成细胞基因治疗提供了一种良好的工具。
The hemagglutinating virus of Japan (HVJ)-liposome method involves the entrapment of DNA and nuclear protein within liposomes and the use of HVJ to enhance liposome fusion with cell membranes. This method has been used successfully for in vivo gene transfer to various types of tissue. In this study, we investigated whether this method transfers genes effectively to normal and malignantly transformed keratinocytes in vivo. We applied HVJ-liposome complex (HLC) containing the beta-galactosidase gene to the tape-stripped skin of hairless rats and detected the enzyme activity in the keratinocytes of the treated skin. Comparison of this method with the naked DNA injection method, which was shown recently to be useful for in vivo gene transfer to keratinocytes, demonstrated that the transfer efficiency of the latter was about 5 times higher than that of the former. We assessed the efficacy of the HVJ-liposome method for gene transfer to transformed keratinocytes by examining the effect of HLC containing the herpes simplex virus thymidine kinase gene on the growth of mouse squamous cell carcinomas. Local injection of HLC into the tumors followed by administration of ganciclovir to mice resulted in tumor growth inhibition. These results indicate that the HVJ-liposome method is suitable for in vivo gene transfer to keratinocytes; also that this method may prove a good tool for basic research into keratinocyte biology and future keratinocyte gene therapy.
表皮：基因治疗的一个有吸引力的靶组织。
DOI：
10.1111/1523-1747.ep12399070
发表时间：
1994
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Greenhalgh,DA;Rothnagel,JA;Roop,DR
通讯作者：
Roop,DR
腺病毒介导的头颈鳞状细胞癌基因治疗。
DOI：
10.1177/000348949610500713
发表时间：
1996
期刊：
The Annals of otology, rhinology, and laryngology
影响因子：
--
作者：
Goebel,EA;Davidson,BL;Zabner,J;Graham,SM;Kern,JA
通讯作者：
Kern,JA