Pathogen evolution in vivo:: Genome dynamics of two isolates obtained 9 years apart from a duodenal ulcer patient infected with a single Helicobacter pylori strain
Pathogen evolution in vivo:: Genome dynamics of two isolates obtained 9 years apart from a duodenal ulcer patient infected with a single Helicobacter pylori strain
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DOI：
10.1128/jcm.43.8.4237-4241.2005
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发表时间：
2005-08-01
影响因子：
9.4
通讯作者：
Ahmed, N
中科院分区：
文献类型：
--
作者：
Prouzet-Mauléon, V;Hussain, MA;Ahmed, N
根除治疗后幽门螺杆菌菌株在胃生态位中的生存和微进化在很大程度上尚未探索。我们分析了从一位接受幽门螺杆菌根除治疗的十二指肠溃疡患者中获得的两个连续分离株的基因组特征。这些分离物基于50个不同的参数进行基因分型，涉及3种不同的指纹识别方法和基因组中几个进化上重要的毒力相关标记，包括9个信息基因位点、cag致病性岛及其右连接、可塑性区簇成员、vacA和iceA等位基因。我们的观察表明，这两种分离株来自同一株，该株在患者身上定殖了近十年，几乎完全相同。然而，在cagA基因及其右连接、vacA m1等位基因和可塑性区簇成员（JHP926）中观察到微进化。这些结果提示幽门螺杆菌具有很强的生存能力，并以微进化的菌株后传形式重新出现，从而提示治疗后患者需要随访。
The survival and microevolution of Helicobacter pylori strains in the niches of the stomach after eradication therapy have largely been unexplored. We analyzed genomic signatures for two successive isolates obtained 9 years apart from a duodenal ulcer patient who underwent eradication therapy for H. pylori. These isolates were genotyped based on 50 different parameters involving three different fingerprinting approaches and several evolutionarily significant and virulence-associated landmarks in the genome, including nine informative gene loci, the cag pathogenicity island and its right junction, members of the plasticity region cluster, and vacA and iceA alleles. Our observations reveal that the two isolates were derived from the same strain that colonized the patient for almost a decade and were almost identical. Microevolution, however, was observed in the cagA gene and its right junction, the vacA m1 allele, and a member of the plasticity region cluster (JHP926). These results suggest that H. pylori has a great ability to survive and reemerge as a microevolved strain posteradication, thereby hinting at the requirement for follow-up of patients after therapy.