Lymphocyte adhesion to psoriatic dermal endothelium is mediated by a tissue-specific receptor/ligand interaction.
Lymphocyte adhesion to psoriatic dermal endothelium is mediated by a tissue-specific receptor/ligand interaction.
复制标题
淋巴细胞与银屑病真皮内皮的粘附是由组织特异性受体/配体相互作用介导的。
DOI：
10.1111/1523-1747.ep12476441
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Chin,YH
中科院分区：
文献类型：
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作者：
Sackstein,R;Falanga,V;Streilein,JW;Chin,YH
皮肤淋巴细胞浸润是银屑病的特征，可能与该病的发病机制有关。我们利用体外淋巴细胞粘附试验来研究介导淋巴细胞向银屑病皮肤迁移的机制。在这个实验中，我们评估了覆盖在正常皮肤和银屑病皮肤冷冻切片上的淋巴细胞的结合。从人血和大鼠胸导管中分离的淋巴细胞对银屑病斑块的真皮内皮具有特异性，但对银屑病患者和正常人的皮肤未受损伤的内皮无特异性；对B细胞和T淋巴细胞亚群结合特性的分析显示，与CD8+ T细胞或B细胞相比，CD4+ T细胞更倾向于结合。这种淋巴细胞-内皮相互作用是一个能量和钙依赖的过程，涉及表面蛋白和碳水化合物部分，需求类似于淋巴组织中淋巴细胞与毛细血管后高内皮小静脉（HEV）相互作用。然而，将淋巴细胞与抗体一起预先孵育，以对抗介导黏附周围淋巴结和肠道相关淋巴组织的HEV的表面分子，不会干扰淋巴细胞与皮肤结合的能力。本研究的结果支持这样的假设，即银屑病真皮中存在的能够介导特异性淋巴细胞-内皮细胞相互作用的特化内皮细胞促进了淋巴细胞从脉管系统向银屑病皮肤的迁移。
Dermal lymphocytic infiltrates are characteristic of psoriasis and may be involved in the pathogenesis of the disease. We have utilized an in vitro lymphocyte adherence assay to examine the mechanism(s) mediating lymphocyte migration into psoriatic skin. In this assay, we assessed the binding of lymphocytes overlaid onto frozen biopsy sections of normal and psoriatic skin. Lymphocytes isolated from human blood and rat thoracic duct hound specifically to dermal endothelia in psoriatic plaques but not to those of uninvolved skin from psoriatic patients or skin from normal individuals; analysis of the binding properties of B cells and T lymphocyte subsets revealed a preferential binding of CD4+ T cells compared with CD8+ T cells or B cells. This lymphocyte-endothelial interaction is an energy- and calcium-dependent process and involves surface protein and carbohydrate moieties, requirements similar to those found in lymphocyte interaction with post-capillary high endothelial venules (HEV) in lymphoid tissues. However, preincubation of lymphocytes with antibodies directed against surface molecules mediating adhesion to HEV of peripheral lymph node and gut-associated lymphoid tissue did not interfere with the capacity of lymphocytes to bind to the skin. The results of this study support the hypothesis that emigration of lymphocytes from vasculature into psoriatic skin is promoted by the presence of specialized endothelia in psoriatic dermis capable of mediating specific lymphocyte-endothelial interactions.