Tissue-specific developmental requirements of Drosophila tyrosine hydroxylase isoforms
Tissue-specific developmental requirements of Drosophila tyrosine hydroxylase isoforms
复制标题
DOI：
10.1002/gene.10178
复制
发表时间：
2003-03-01
期刊：
影响因子：
1.5
通讯作者：
Birman, S
中科院分区：
文献类型：
--
作者：
Friggi-Grelin, F;Iché, M;Birman, S
果蝇酪氨酸羟化酶（Drosophila tyrosine hydroxylase， DTH）是多巴胺（DA）生物合成的关键酶，在神经和皮下DA合成细胞中表达。我们之前报道了两种DTH同种异构体在果蝇中通过组织特异性选择性剪接产生，显示出不同的调节特性。我们现在有选择地在体内苍白（饼，即DTH缺陷）突变背景下表达每个DTH亚型。我们发现，在所有DA细胞中，DTH的下皮层异构体的表达可以挽救pie的胚胎致命性，但神经异构体却不能，这表明下皮层异构体是果蝇角质层生物合成和存活所绝对需要的。此外，我们报告了关于DTH在中枢神经系统和皮下过表达的后果的新观察结果。我们的研究结果提供了证据，证明DTH基因的组织特异性选择性剪接是果蝇发育的重要过程。(C) 2003 Wiley-Liss, Inc。
Drosophila tyrosine hydroxylase (DTH) is a key enzyme in dopamine (DA) biosynthesis, which is expressed in neural and hypodermal DA-synthesizing cells. We previously reported that two DTH isoforms are produced in flies through tissue-specific alternative splicing that show distinct regulatory properties. We have now selectively expressed each DTH isoform in vivo in a pale (pie, i.e., DTH-deficient) mutant background. We show that the embryonic lethality of pie can be rescued by expression of the hypodermal, but not the neural, DTH isoform in all DA cells, indicating that the hypoderm- isoform is absolutely required for cuticle biosynthesis and survival in Drosophila. In addition, we report new observations on the consequences of DTH overexpression in the CNS and hypoderm. Our results provide evidence that tissue-specific alternative splicing of the DTH gene is a vital process in Drosophila development. (C) 2003 Wiley-Liss, Inc.