Ectopic expression of CsMADS24, an AGAMOUS ortholog from cucumber, causes a homeotic conversion of sepals into carpels in transgenic Arabidopsis plants
Ectopic expression of CsMADS24, an AGAMOUS ortholog from cucumber, causes a homeotic conversion of sepals into carpels in transgenic Arabidopsis plants
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CsMADS24（一种来自黄瓜的 AGAMOUS 直系同源物）的异位表达导致转基因拟南芥植物中萼片同源转化为心皮
DOI：
10.2298/abs180528037z
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发表时间：
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影响因子：
0.8
通讯作者：
Shiqiang Liu
中科院分区：
文献类型：
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作者：
Yong Zhou;Lifang Hu;Lingli Ge;Guanghua Li;Peng He;Lunwei Jiang;Shiqiang Liu
拟南芥花同源c功能MADS基因AGAMOUS （AG）。在确定雄蕊和心皮身份以及决定花分生组织中起关键作用。然而，关于花同源c功能基因的研究很少。黄瓜到目前为止。在本研究中，CsMADS24是黄瓜中一个假定的AG同源物。被分离并鉴定。序列分析和蛋白序列比对。推断出的CsMADS24蛋白含有典型的MIKC结构。n端延伸，以及两个高度保守的AG基序（I和II）。系统发育。分析表明，CsMADS24属于核心诊断分支，但与核心诊断分支距离较远。基生单子植物、单子植物和裸子植物的AG同源物。表达式分析。RT-PCR结果显示，CsMADS24仅在雌花芽中表达。在原地。杂交结果显示，CsMADS24仅在心皮中表达。功能分析表明，部分萼片已转化为类果皮。35S::35S::CsMADS24转基因植株的结构。此外，在花萼发育过程中也观察到开花提前和花器官脱落延迟的现象。转基因拟南芥。我们的研究结果表明，AG同源物起着排他性的作用。为揭示黄瓜心皮发育机制提供依据。黄瓜的生殖发育。
The floral homeotic C-function MADS gene AGAMOUS (AG) in Arabidopsis .plays crucial roles in specifying stamen and carpel identities as well as determining floral .meristem. However, there have been only a few studies of floral homeotic C-function genes in .cucumber thus far. In the present study, CsMADS24, a putative AG ortholog from cucumber, .was isolated and characterized. Sequence analysis and protein sequence alignment revealed .that the deduced CsMADS24 protein contained the typical MIKC structure and the .N-terminal extension, as well as two highly conserved AG motifs (I and II). Phylogenetic .analysis showed that CsMADS24 fell into the clade of core eudicots, while being distant from .the AG orthologs of basal eudicots, monocots and gymnosperms. Expression analysis by .RT-PCR showed that CsMADS24 was exclusively expressed in female flower buds. In situ .hybridization revealed that CsMADS24 expression was only detected in the carpels. .Functional analyses indicated that the sepals were partly converted into carpelloid-like .structures in 35S::35S::CsMADS24 transgenic plants. In addition, earlier flowering and .delayed floral organ abscission during the development of siliques were also observed in .transgenic Arabidopsis. Our findings demonstrate that the AG ortholog plays an exclusive role .in carpel specification of cucumber, providing a basis for revealing the mechanisms of .reproductive development in cucumber.