The exocyst is a Ral effector complex
The exocyst is a Ral effector complex
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DOI：
10.1038/ncb728
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发表时间：
2002-01-01
影响因子：
21.3
通讯作者：
White, MA
中科院分区：
文献类型：
--
作者：
Moskalenko, S;Henry, DO;White, MA
细胞质囊泡传递到离散的质膜域对于建立和维持细胞极性、神经突分化和调节胞吐作用至关重要。囊泡是一个多亚单位复合体，需要载体靶向的一个子集的分泌囊。哺乳动物调节这种复合物活性的机制尚不清楚。在这里，我们表明Sec5是囊泡的一个组成部分，是激活的Ral GTPases的直接靶标。Ral gtpase调节上皮细胞中基底外侧蛋白的靶向，神经内分泌细胞中分泌依赖性的胞外分泌和胞外复合物的组装。这些观察结果将rgtp酶定义为囊泡运输的关键调节因子。
Delivery of cytoplasmic vesicles to discrete plasma-membrane domains is critical for establishing and maintaining cell polarity, neurite differentiation and regulated exocytosis. The exocyst is a multisubunit complex required for vectorial targeting of a subset of secretory vesicles. Mechanisms that regulate the activity of this complex in mammals are unknown. Here we show that Sec5, an integral component of the exocyst, is a direct target for activated Ral GTPases. Ral GTPases regulate targeting of basolateral proteins in epithelial cells, secretagogue-dependent exocytosis in neuroendocrine cells and assembly of exocyst complexes. These observations define Ral GTPases as critical regulators of vesicle trafficking.