Molecular detection of fungal communities in the Hawaiian marine sponges Suberites zeteki and Mycale armata
Molecular detection of fungal communities in the Hawaiian marine sponges Suberites zeteki and Mycale armata
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DOI：
10.1128/aem.01315-08
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发表时间：
2008-10-01
影响因子：
4.4
通讯作者：
Wang, Guangyi
中科院分区：
文献类型：
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作者：
Gao, Zheng;Li, Binglin;Wang, Guangyi
共生微生物在宿主的健康和栖息地范围中发挥着各种重要作用。虽然海绵中的原核生物已被广泛表征，但海绵栖息真菌的多样性几乎没有使用分子方法进行探索。真菌是许多海洋和陆地生态系统的重要组成部分，它们可能是海绵-微生物相互作用中具有重要生态意义的类群。本研究测试了使用现有的真菌引物进行海绵相关真菌群落分子分析的可行性。在真菌rRNA基因或ITS克隆文库构建中，所选的8对引物均未获得满意的结果。然而，10个变性梯度凝胶电泳（DGGE）引物组，这是专为从陆地环境样品中优先扩增真菌rRNA基因或ITS区，3成功地从海绵中的真菌目标扩增。DGGE分析表明，不同种类的海绵（Suberites zeteki和Mycale armata）之间的真菌群落存在差异，海绵和海水之间也存在差异。DGGE谱带序列分析分别鉴定出23种和21种真菌。zeteki和M. Armata，分别。这些种代表了11个分类目，属于子囊菌门（7个目）和担子菌门（4个目）。其中五个分类目（马拉色目、皮质目、多孔菌目、伞菌目和盾壳菌目和不定毛壳菌目）现在首次在海绵中被鉴定。从S. zeteki和M. armata，分别是潜在的新物种，因为它们的低序列同一性（
Symbiotic microbes play a variety of fundamental roles in the health and habitat ranges of their hosts. While prokaryotes in marine sponges have been broadly characterized, the diversity of sponge-inhabiting fungi has barely been explored using molecular approaches. Fungi are an important component of many marine and terrestrial ecosystems, and they may be an ecologically significant group in sponge-microbe interactions. This study tested the feasibility of using existing fungal primers for molecular analysis of sponge-associated fungal communities. None of the eight selected primer pairs yielded satisfactory results in fungal rRNA gene or internal transcribed spacer (ITS) clone library constructions. However, 3 of 10 denaturing gradient gel electrophoresis (DGGE) primer sets, which were designed to preferentially amplify fungal rRNA gene or ITS regions from terrestrial environmental samples, were successfully amplified from fungal targets in marine sponges. DGGE analysis indicated that fungal communities differ among different sponge species (Suberites zeteki and Mycale armata) and also vary between sponges and seawater. Sequence analysis of DGGE bands identified 23 and 21 fungal species from each of the two sponge species S. zeteki and M. armata, respectively. These species were representatives of 11 taxonomic orders and belonged to the phyla of Ascomycota (seven orders) and Basidiomycota (four orders). Five of these taxonomic orders (Malasseziales, Corticiales, Polyporales, Agaricales, and Dothideomycetes et Chaetothyriomcetes incertae sedis) have now been identified for the first time in marine sponges. Seven and six fungal species from S. zeteki and M. armata, respectively, are potentially new species because of their low sequence identity (