Genetically Encoded Copper(I) Reporters with Improved Response for Use in Imaging
Genetically Encoded Copper(I) Reporters with Improved Response for Use in Imaging
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基因编码的铜 (I) 报告基因具有改进的响应，可用于成像。
DOI：
10.1021/ja3106779
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发表时间：
2013-02-27
影响因子：
15
通讯作者：
He, Chuan
中科院分区：
文献类型：
--
作者：
Liu, Jun;Karpus, Jason;He, Chuan
铜是最重要的生物金属离子之一，因为它在许多蛋白质中起催化辅因子的作用。然而，过量的铜是剧毒的。因此，铜是高度调节的，并且铜稳态由各种生物体中的许多金属调节蛋白控制。在这里，我们报告了一种基因编码的铜（I）探针能够监测活细胞内的铜波动。我们将铜调节蛋白Ace1插入到黄色荧光蛋白中，其选择性地结合铜（I）并产生改进的铜（I）探针。
Copper represents one of the most important biological metal ions due to its role as a catalytic cofactor in a multitude of proteins. However, an excess of copper is highly toxic. Thus, copper is heavily regulated, and copper homeostasis is controlled by many metalloregulatory proteins in various organisms. Here we report a genetically encoded copper(I) probe capable of monitoring copper fluctuations inside living cells. We insert the copper regulatory protein Ace1 into a yellow fluorescent protein, which selectively binds copper(I) and generates improved copper(I) probes.