C-MYB REPRESSION OF C-ERBB-2 TRANSCRIPTION BY DIRECT BINDING TO THE C-ERBB-2 PROMOTER
C-MYB REPRESSION OF C-ERBB-2 TRANSCRIPTION BY DIRECT BINDING TO THE C-ERBB-2 PROMOTER
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DOI：
10.1074/jbc.270.16.9384
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发表时间：
1995-04-21
影响因子：
4.8
通讯作者：
ISHII, S
中科院分区：
文献类型：
--
作者：
MIZUGUCHI, G;KANEIISHII, C;ISHII, S
The c-myb proto oncogene product (c-Myb) is a transcriptional activator that can bind to the specific DNA sequences. Although c-Myb also represses an artificial promoter containing the Myb binding sites, natural target genes transcriptionally repressed by c-Myb have not been identified. We have found that the human c-erbB-a promoter activity is repressed by c-Myb or B-Myb in a chloramphenicol acetyltransferase co-transfection assay. Domain analyses of c-Myb suggested that Myb re presses the c-erbB-a promoter activity by competing with positive regulators of the c-erbB-2 promoter. In in vitro transcription assays, Myb proteins containing only the DNA binding domain could repress c-erbB-2 promoter activity. Two Myb binding sites in the c-erbB-2 promoter were critical for transcriptional repression by c-Myb. One of the two Myb binding sites overlaps the TATA box, and DNase I footprint analyses indicated that c-Myb can compete with TFIID. These results suggest that Myb-induced trans-repression of the c-erbB-2 promoter partly involves competition between Myb and TFIID.
