Single-cell phenomics reveals intra-species variation of phenotypic noise in yeast.
Single-cell phenomics reveals intra-species variation of phenotypic noise in yeast.
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DOI：
10.1186/1752-0509-7-54
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发表时间：
2013-07-03
影响因子：
--
通讯作者：
Ohya Y
中科院分区：
文献类型：
--
作者：
Yvert G;Ohnuki S;Nogami S;Imanaga Y;Fehrmann S;Schacherer J;Ohya Y
表型性状的大多数定量测量代表大量细胞的宏观贡献。然而，一个组织的细胞的行为并不相似，对几种生物体的分子研究表明，调控可能是高度随机的，有时会在组织内产生多样化的细胞表型。表型噪音，在这里定义为性状变异之间的同基因细胞类型和共享一个共同的环境，因此受到了很多关注。考虑到在波动环境中表型噪声提供的潜在适应优势，正在考虑直接进行进化选择的可能性。为了使选择发挥作用，表型噪音必须在当代基因型之间有所不同。然而，这种情况是否存在尚不清楚，因为表型噪音在自然种群中很少被量化。使用自动图像分析，我们描述了S。酿酒酵母的形态在单细胞分辨率。我们分析了37个自然菌株的1,000多个细胞中的数百个数量性状，这些菌株代表了该物种的各种地理和生态起源。我们观察到丰富的菌株之间的性状变异，与它们的生态起源或人口的历史。表型噪声强烈依赖于菌株背景。噪声变化主要是特征特异性的（特定菌株的特征子集的噪声升高），但也是全球性的（一些菌株的许多不相关的特征噪声升高）。我们的研究结果表明，表型噪声在自然种群之间确实存在数量上的差异。这支持了一种可能性，即如果噪音是适应性的，那么微进化可能会在野外调整它。这种调整可能发生在特定的性状或通过改变全局表型缓冲的程度。
Most quantitative measures of phenotypic traits represent macroscopic contributions of large numbers of cells. Yet, cells of a tissue do not behave similarly, and molecular studies on several organisms have shown that regulations can be highly stochastic, sometimes generating diversified cellular phenotypes within tissues. Phenotypic noise, defined here as trait variability among isogenic cells of the same type and sharing a common environment, has therefore received a lot of attention. Given the potential fitness advantage provided by phenotypic noise in fluctuating environments, the possibility that it is directly subjected to evolutionary selection is being considered. For selection to act, phenotypic noise must differ between contemporary genotypes. Whether this is the case or not remains, however, unclear because phenotypic noise has very rarely been quantified in natural populations. Using automated image analysis, we describe here the phenotypic diversity of S. cerevisiae morphology at single-cell resolution. We profiled hundreds of quantitative traits in more than 1,000 cells of 37 natural strains, which represent various geographical and ecological origins of the species. We observed abundant trait variation between strains, with no correlation with their ecological origin or population history. Phenotypic noise strongly depended on the strain background. Noise variation was largely trait-specific (specific strains showing elevated noise for subset of traits) but also global (a few strains displaying elevated noise for many unrelated traits). Our results demonstrate that phenotypic noise does differ quantitatively between natural populations. This supports the possibility that, if noise is adaptive, microevolution may tune it in the wild. This tuning may happen on specific traits or by varying the degree of global phenotypic buffering.
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9.8
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