The acute regulation of mitochondrial proton conductance in cells and mitochondria from the brown fat of cold-adapted and warm-adapted guinea pigs.
The acute regulation of mitochondrial proton conductance in cells and mitochondria from the brown fat of cold-adapted and warm-adapted guinea pigs.
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冷适应和热适应豚鼠棕色脂肪细胞和线粒体中线粒体质子电导的急性调节。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
David G. Nicholls
中科院分区：
文献类型：
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作者：
R. Locke;Eduardo Rial;David G. Nicholls
细胞和线粒体是从适应 4-7 摄氏度或 22-25 摄氏度的成年豚鼠的棕色脂肪组织中制备的。冷适应细胞表现出去甲肾上腺素刺激的、普萘洛尔敏感的呼吸，但去甲肾上腺素未能增加热适应细胞的呼吸。冷适应线粒体中的嘌呤核苷酸敏感质子电导高于热适应对照。同时，冷适应线粒体对内源性和注入脂肪酸的解偶联效应极其敏感，并且类似于来自冷适应仓鼠棕色脂肪组织的线粒体。热适应线粒体的敏感性要低九倍，并且与肝线粒体相似。对于冷适应而非热适应的线粒体，呼吸作用与脂肪酸输注速率成比例增加。结论是，32000-Mr质子电导途径的存在对于表达对脂肪酸解偶联的高敏感性是必要的，这表明脂肪酸直接与该蛋白质相互作用以在产热的急性调节过程中调节质子电导。
Cells and mitochondria were prepared from the brown adipose tissue of adult guinea-pigs adapted to either 4-7 degrees C or 22-25 degrees C. The cold-adapted cells displayed noradrenaline-stimulated, propranolol-sensitive respiration, but noradrenaline failed to increase the respiration of the warm-adapted cells. Purine-nucleotide-sensitive proton conductance was greater in cold-adapted mitochondria than in warm-adapted controls. At the same time cold-adapted mitochondria were extremely sensitive to the uncoupling effect of endogenous and infused fatty acids, and resembled the mitochondria from the brown adipose tissue of cold-adapted hamsters. Warm-adapted mitochondria were ninefold less sensitive, and resembled liver mitochondria. With cold-adapted, but not warm-adapted mitochondria, respiration increased proportionately to the rate of fatty acid infusion. It is concluded that the presence of the 32000-Mr proton conductance pathway is necessary for the expression of a high sensitivity to fatty acid uncoupling, suggesting that the fatty acids interact directly with this protein to modulate the proton conductance during the acute regulation of thermogenesis.