Preparative Separation and Amino Acid Composition of Neurofilament Triplet Proteins
Preparative Separation and Amino Acid Composition of Neurofilament Triplet Proteins
复制标题
神经丝三联体蛋白的制备分离和氨基酸组成
DOI：
10.1111/j.1471-4159.1982.tb10861.x
复制
发表时间：
1982
影响因子：
4.7
通讯作者：
W. Schlaepfer
中科院分区：
文献类型：
--
作者：
R. Hogue;H.‐L. Wu;W. Schlaepfer
Abstract: Intact neurofilaments were isolated from bovine spinal cord white matter, washed by sedimentation in 0.1 m‐NaCl, and extracted with 8 m‐urea. Solubilized neurofilament triplet proteins of molecular weights approximately 68,000 (P68), 150,000 (P150), and 200,000 (P200) were purified by preparative electrophoresis, using an LKB 7900 Uniphor apparatus. The method provides for an enhanced yield of purified protein and has markedly reduced admixture of electrophoresed protein with acrylamide and associated protein contaminants. Amino acid compositions of the purified neurofilament triplet proteins are reported and compared.
