Peonidin inhibits phorbol-ester-induced COX-2 expression and transformation in JB6 P+ cells by blocking phosphorylation of ERK-1 and -2
Peonidin inhibits phorbol-ester-induced COX-2 expression and transformation in JB6 P+ cells by blocking phosphorylation of ERK-1 and -2
复制标题
DOI：
10.1196/annals.1397.055
复制
发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Lee, Hyong Joo
中科院分区：
文献类型：
--
作者：
Kwon, Jung Yeon;Lee, Ki Won;Lee, Hyong Joo
环氧化酶-2（考克斯-2）的异常上调在各种类型的转化细胞和癌细胞中经常被观察到。许多抗炎剂已被证明通过靶向考克斯-2（一种参与炎症过程的限速酶）发挥化学预防作用。花青素是天然存在的多酚化合物，赋予各种水果，蔬菜和植物强烈的颜色。牡丹色素是另一种具有代表性的花青素，但其化学预防潜力尚未完全阐明。本文研究了芍药苷对12-O-十四酰佛波醇-13-乙酸酯（TPA）诱导的小鼠表皮细胞（JB 6 P+细胞）考克斯-2表达和转化的影响。芍药苷处理的JB 6 P+细胞抑制TPA诱导的考克斯-2的表达，也减少TPA诱导的肿瘤转化和阻断TPA诱导的细胞外信号调节激酶（ERK）的磷酸化。抑制调节ERK激活的信号传导机制强烈表明，芍药苷表现出化学预防以及抗炎活性。
Abnormal upregulation of cyclooxygenase-2 (COX-2) has been frequently observed in various types of transformed and cancerous cells. Numerous anti-inflammatory agents have been shown to exert chemopreventive effects by targeting COX-2, a rate-limiting enzyme involved in the inflammatory process. Anthocyanins are naturally occurring polyphenolic compounds that endow various fruits, vegetables, and plants with intense colors. Peonidin is another representative anthocyanidin, but its chemopreventive potential has not been fully described. This article investigated the effect of peonidin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced COX-2 expression and transformation in JB6 P+ mouse epidermal cells (JB6 P+ cells). Treatment of JB6 P+ cells with peonidin inhibited TPA-induced COX-2 expression, and also decreased TPA-induced neoplastic transformation and blocked TPA-induced phosphorylation of extracellular signal-regulated kinases (ERKs) in the cells. The inhibition of the signaling mechanism regulating the activation of ERKs strongly suggests that peonidin exhibits chemopreventive as well as anti-inflammatory activities.