Synthesis of single-walled carbon nanotubes–chitosan nanocomposites for the development of an electrochemical biosensor for serum leptin detection
Synthesis of single-walled carbon nanotubes–chitosan nanocomposites for the development of an electrochemical biosensor for serum leptin detection
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DOI：
10.1016/j.matlet.2017.10.036
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发表时间：
2018-01
影响因子：
3
通讯作者：
Qiongyuan Zhang;Ying Qing;Xinyi Huang;Chaorui Li;Jianjiang Xue
中科院分区：
文献类型：
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作者：
Qiongyuan Zhang;Ying Qing;Xinyi Huang;Chaorui Li;Jianjiang Xue
Single-walled carbon nanotubes (SWCNTs) cannot be stably dispersed in aqueous solutions, which greatly limit their use in biomedical applications. In this study, functionalized SWCNTs were dispersed in chitosan (CS) solution, which greatly promoted the water solubility of SWCNTs and formed a uniformly dispersed solution. The use of EDC/NHS crosslinking solution to activate carboxyl groups into amino groups, greatly improved the affinity of SWCNTs for biomolecules. TEM characterization of the nanocomposites showed that SWCNTs were uniformly dispersed in CS solution. Cyclic voltammetry and electrochemical impedance spectroscopy revealed that the nanocomposites covering the glassy carbon electrode (GCE) greatly improved the conductivity of the electrodes and enhanced the electron transfer rate. A simple electrochemical biosensor for the detection of serum leptin was successfully fabricated with the functionalized-SWCNTs–CS biopolymer nanocomposites on GCEs. The biosensor demonstrated a wide linear range (0–1000 ng mL−1) and a detection limit of 5 pg mL−1(S/N = 3).
