An Official American Thoracic Society Workshop Report: Features and Measurements of Experimental Acute Lung Injury in Animals
An Official American Thoracic Society Workshop Report: Features and Measurements of Experimental Acute Lung Injury in Animals
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DOI：
10.1165/rcmb.2009-0210st
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发表时间：
2011-05-01
影响因子：
6.4
通讯作者：
Villar, Jesus
中科院分区：
文献类型：
--
作者：
Matute-Bello, Gustavo;Downey, Gregory;Villar, Jesus
人类急性肺损伤（ALI）有明确的定义，但对于动物模型中急性肺损伤的主要特征尚未达成一致。成立了一个委员会来确定动物模型中 ALI 的主要特征，并确定评估这些特征的最相关方法。我们使用了德尔菲法，向实验性肺损伤专家小组分发了一系列调查问卷。委员会得出的结论是，实验性 ALI 的主要特征包括组织损伤的组织学证据、肺泡毛细血管屏障的改变、炎症反应的存在以及生理功能障碍的证据；他们建议，要确定是否发生了 ALI，至少应出现 ALI 的这四个主要特征中的三个。委员会还为 ALI 的每个主要特征确定了关键的“非常相关”和“部分相关”测量，并建议使用至少一项“非常相关”测量，最好使用一到两个额外的单独测量来确定是否存在 ALI 的主要特征。最后，委员会强调，并非所有列出的测量都可以或应该在每项研究中进行，并且列表中未包含的测量绝不是“无关紧要的”。我们的 ALI 特征和测量列表旨在为研究人员提供指导，最终研究人员应选择最适合所解决的实验问题的特定测量，并考虑实验设计的任何独特方面。
Acute lung injury (ALI) is well defined in humans, but there is no agreement as to the main features of acute lung injury in animal models. A Committee was organized to determine the main features that characterize ALI in animal models and to identify the most relevant methods to assess these features. We used a Delphi approach in which a series of questionnaires were distributed to a panel of experts in experimental lung injury. The Committee concluded that the main features of experimental ALI include histological evidence of tissue injury, alteration of the alveolar capillary barrier, presence of an inflammatory response, and evidence of physiological dysfunction; they recommended that, to determine if ALI has occurred, at least three of these four main features of ALI should be present. The Committee also identified key "very relevant" and "somewhat relevant" measurements for each of the main features of ALI and recommended the use of least one "very relevant" measurement and preferably one or two additional separate measurements to determine if a main feature of ALI is present. Finally, the Committee emphasized that not all of the measurements listed can or should be performed in every study, and that measurements not included in the list are by no means "irrelevant." Our list of features and measurements of ALI is intended as a guide for investigators, and ultimately investigators should choose the particular measurements that best suit the experimental questions being addressed as well as take into consideration any unique aspects of the experimental design.