Enumeration of T and B lymphocytes in bovine leukemia virus-infected cattle, using monoclonal antibodies.
Enumeration of T and B lymphocytes in bovine leukemia virus-infected cattle, using monoclonal antibodies.
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使用单克隆抗体对感染牛白血病病毒的牛进行 T 和 B 淋巴细胞计数。
DOI：
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发表时间：
1988
影响因子：
1
通讯作者：
W. Davis
中科院分区：
文献类型：
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作者：
D. Williams;G. Amborski;W. Davis
用单抗和微量荧光法测定了牛白血病病毒(BLV)感染奶牛血液中B、T淋巴细胞的绝对数。BLV感染奶牛的外周血淋巴细胞数量显著高于BLV阴性奶牛。在3头感染BLV的非淋巴细胞奶牛中，淋巴细胞数量的增加归因于T淋巴细胞数量的显著增加；在3头持续感染BLV的奶牛中，淋巴细胞数量的增加归因于B和T淋巴细胞数量的显著增加。一头持续存在淋巴细胞增多症的奶牛的淋巴细胞数量很高，这头奶牛的淋巴细胞中含有的细胞似乎染上了牛B和T淋巴细胞的特异性标记。我们的结论是，牛感染B细胞嗜淋巴逆转录病毒，不仅影响B细胞，也影响T细胞。
Monoclonal antibodies and microfluorimetry were used to determine the absolute number of B and T lymphocytes in the blood of bovine leukemia virus (BLV)-infected cows. The blood lymphocyte populations from BLV-infected cows were significantly higher than those from BLV-negative cows. The increase in the lymphocyte population in 3 BLV-infected nonlymphocytotic cows was attributed to a significant increase in the number of T lymphocytes; in 3 BLV-infected persistently lymphocytotic cows, the increase was attributed to a significant increase in the number of B and T lymphocytes. One persistently lymphocytotic cow had a high lymphocyte count, and lymphocytes from this cow contained cells that appeared to stain with markers specific for bovine B and T lymphocytes. We concluded that infection of cattle with the B-cell lymphotropic retrovirus, BLV, not only affected B cells, but also T cells.