Intracellular Occurrence of ε-Proteobacterial 16S rDNA Sequences in the Vestimentiferan Trophosome
Intracellular Occurrence of ε-Proteobacterial 16S rDNA Sequences in the Vestimentiferan Trophosome
复制标题
Vesimentiferan 营养体中 ε-变形菌 16S rDNA 序列的细胞内出现
DOI：
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发表时间：
1997
影响因子：
2.3
通讯作者：
K. Horikoshi
中科院分区：
文献类型：
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作者：
T. Naganuma;C. Kato;H. Hirayama;N. Moriyama;J. Hashimoto;K. Horikoshi
管虫的内共生体通常被认为是γ-变形菌的硫氧化剂。我们已经鉴定了两个新的16S核糖体RNA基因（rDNA），可能是e-变形杆菌，从滋养体内容的veestimentiferan管蠕虫。系统发育分析表明，16S rnas与Arcobacter的rnas密切相关，其中大多数已知与动物宿主相关，但非化学合成。16S rnas的细胞内定位通过使用其特异性探针进行原位杂交证实。这是首次报道与肠管虫相关的e-Proteobacterial 16S rnas。
Endosymbionts of vestimentiferan tubeworms are generally thought to be γ-Proteobacterial sulfur oxidizers. We have identified two novel 16S ribosomal RNA genes (rDNA), presumably of e-Proteobacteria, from the trophosome content of a vestimentiferan tubeworm. Phylogenetic analysis suggested that the 16S rDNAs were closely related to those of Arcobacter, most of which are known to be associated with animal hosts but are non-chemosynthetic. Intracellular localizations of the 16S rDNAs were confirmed by in situ hybridization using their specific probes. This is the first report of e-Proteobacterial 16S rDNAs associated with a vestimentiferan tubeworm.