Temperature-dependent activation of ERK/MAPK in yolk cells and its role in embryonic diapause termination in the silkworm Bombyx mori
Temperature-dependent activation of ERK/MAPK in yolk cells and its role in embryonic diapause termination in the silkworm Bombyx mori
复制标题
DOI：
10.1016/j.jinsphys.2005.07.009
复制
发表时间：
2005-12-01
影响因子：
2.2
通讯作者：
Fujiwara, Y
中科院分区：
文献类型：
--
作者：
Iwata, K;Shindome, C;Fujiwara, Y
家蚕需要2-3个月的低温（5 ℃）来终止胚胎滞育.然而，其分子机制尚不清楚。细胞外信号调节激酶（ERK）和丝裂原活化蛋白激酶（MAPK）在滞育卵的卵黄细胞中被激活，这与胚胎在25 ℃获得发育能力是一致的。在5 ℃孵育45天的滞育卵中，卵黄细胞颗粒化和解离也开始。利用去绒卵培养作为解除滞育的模型系统，观察到MAPK-ERK激酶（MEK）抑制剂U 0126对卵黄细胞的解离和胚胎发育均具有抑制作用。因此，我们认为卵黄细胞中的ERK在调控B胚胎滞育的形态变化和解除滞育中起作用。森。(c)2005爱思唯尔有限公司保留所有权利。
The silkworm Bombyx mori requires 2-3 months of low temperature (5 C) to terminate embryonic diapause. The molecular mechanisms, however, are unknown. Extracellular signal-regula led kinase (ERK) mitogen-activated protein kinase (MAPK) is temperature-dependently activated in the yolk cells of diapausing eggs after 45 days Lit 5 degrees C, coincident with the acquisition of developmental competence of the embryos at 25 C. Yolk cell granulation and dissociation also begin in diapause eggs incubated at 5 degrees C for 45 days. We used dechorionated egg culture as a model system of diapause termination and observed that both yolk cell dissociation and embryonic development are inhibited by MAPK-ERK kinase (MEK) inhibitor U0126, Therefore, we suggest that ERK in yolk cells has a role in regulating changes in yolk morphology and termination of embryonic diapause in B. mori. (c) 2005 Elsevier Ltd. All rights reserved.