Quality of long-term cryopreserved umbilical cord blood units for hematopoietic cell transplantation
Quality of long-term cryopreserved umbilical cord blood units for hematopoietic cell transplantation
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DOI：
10.1007/s12185-010-0755-x
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发表时间：
2011-01-01
影响因子：
2.1
通讯作者：
Isoyama, Keiichi
中科院分区：
文献类型：
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作者：
Yamamoto, Shohei;Ikeda, Hirokazu;Isoyama, Keiichi
本研究的目的是评估用于造血细胞移植（HCT）的长期冷冻脐带血（CB）单位的质量。检查了冷冻保存 10 年用于 HCT 的 CB 单位的总有核细胞 (TNC)、造血祖细胞 (HPC；CD34+ 细胞、集落形成单位 - 粒细胞/巨噬细胞 [CFU-GM]) 的恢复情况以及活细胞、CD34+ CD38- 细胞和 CD34+ CXCR4+ 细胞的百分比。对分别冷冻保存10年（作为研究组）和1个月（作为对照组）的18个CB单位进行了分析。研究组TNC、CD34+细胞和CFU-GMs的回收率为88.72+/-16.40、68.39+/-18.37和42.28+/-38.16%，对照组为80.17+/-14.46、72.67+/-20.38和49.61+/-36.39%组（分别为 p = 0.106、p = 0.513 和 p = 0.559）。研究组与对照组TNC、CD34+细胞及CFU-GMs的恢复率无显着差异。研究组解冻后活细胞、CD34+ CD38- 细胞和 CD34+ CXCR4+ 细胞的平均基础百分比分别为 83.69 +/- 9.45、9.11 +/- 4.13 和 81.65 +/- 10.82%。这些结果表明，长期冷冻保存不会对 HCT CB 单位的质量产生负面影响。
The aim of this study was to evaluate the quality of long-term cryopreserved umbilical cord blood (CB) units for hematopoietic cell transplantation (HCT). The recovery of the number of total nucleated cell (TNC), hematopoietic progenitor cells (HPCs; CD34+ cells, colony-forming units-granulocyte/macrophages [CFU-GMs]), and the percentage of viable cells, CD34+ CD38- cells, and CD34+ CXCR4+ cells of CB units cryopreserved for 10 years for HCT were examined. Eighteen CB units cryopreserved for 10 years (as the study group) and for 1 month (as the control group), respectively, were analyzed. The recovery rate of TNC, CD34+ cells and CFU-GMs were 88.72 +/- 16.40, 68.39 +/- 18.37 and 42.28 +/- 38.16% for the study group and 80.17 +/- 14.46, 72.67 +/- 20.38 and 49.61 +/- 36.39% for the control group (p = 0.106, p = 0.513 and p = 0.559, respectively). There were no significant differences in the recovery rate of TNC, CD34+ cells and CFU-GMs between the study group and the control group. The mean basal percentage of viable cells, CD34+ CD38- cells, and CD34+ CXCR4+ cells after thawing were 83.69 +/- 9.45, 9.11 +/- 4.13 and 81.65 +/- 10.82% for the study group. These results indicate that long-term cryopreservation does not negatively affect the quality of CB units for HCT.