Identification and characterisation of Dof transcription factors in the cucumber genome.
Identification and characterisation of Dof transcription factors in the cucumber genome.
复制标题
黄瓜基因组中 Dof 转录因子的鉴定和表征
DOI：
10.1038/srep23072
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发表时间：
2016-03-16
影响因子：
4.6
通讯作者：
Xu Y
中科院分区：
文献类型：
--
作者：
Wen CL;Cheng Q;Zhao L;Mao A;Yang J;Yu S;Weng Y;Xu Y
黄瓜易受许多叶部病害的侵害。最近的研究报道了黄瓜几种疾病的候选基因的克隆，但确切的防御机制仍不清楚。Dof基因在植物的生长发育和对生物和非生物胁迫的响应中起着重要作用，编码植物特异性转录因子的Dof基因可以促进防御相关基因在全基因组水平上的大规模表达。该家族中的基因已经在几种植物物种中被鉴定和表征，但在黄瓜中没有。在本研究中，我们确定了36 CsDof成员从黄瓜草案基因组，可分为8组。对CsDoffamily基因的比例、重复事件、染色体定位、顺式元件和miRNA靶位点进行了全面的研究。因此，我们分析了CsDof基因在特定组织中的表达模式及其对两种生物胁迫（西瓜花叶病毒和霜霉病）的反应。这些结果表明CsDof可能参与了黄瓜对生物胁迫的抗性。
Cucumber is vulnerable to many foliage diseases. Recent studies reported cloning of candidate genes for several diseases in cucumber; however, the exact defence mechanisms remain unclear. Dof genes have been shown to play significant roles in plant growth, development and responses to biotic and abiotic stresses.Dofgenes coding for plant-specific transcription factors can promote large-scale expression of defence-related genes at whole genome level. The genes in the family have been identified and characterized in several plant species, but not in cucumber. In the present study, we identified 36CsDofmembers from the cucumber draft genomes which could be classified into eight groups. The proportions of theCsDoffamily genes, duplication events, chromosomal locations, cis-elements and miRNA target sites were comprehensively investigated. Consequently, we analysed the expression patterns ofCsDofgenes in specific tissues and their response to two biotic stresses (watermelon mosaic virus and downy mildew). These results indicated thatCsDofmay be involved in resistance to biotic stresses in cucumber.