Differential gene expression profiles of whole lesions from patients with oral lichen planus
Differential gene expression profiles of whole lesions from patients with oral lichen planus
复制标题
口腔扁平苔藓患者整个病变的差异基因表达谱。
DOI：
10.1111/j.1600-0714.2009.00764.x
复制
发表时间：
2009-05-01
影响因子：
3.3
通讯作者：
Cheng, Bin
中科院分区：
文献类型：
--
作者：
Tao, Xiao-An;Li, Chun-Yang;Cheng, Bin
口腔扁平苔藓（OLP）的分子生物学特性在很大程度上是未知的。本研究的目的是利用DNA微阵列技术在基因组尺度上鉴定其发病机制的基因。采用Affymetrix基因芯片对9例OLP患者和9例对照组的RNA样本进行分析。实时逆转录聚合酶链反应（RT-PCR）分析证实genecchip结果。共鉴定出985个差异表达基因，其中上调629个，下调356个。这些基因参与了许多功能分类和生化途径。FOXP3、VEGFA、ANGPT1、MMP1和SCGB2A2的定量RT-PCR分析结果与基因芯片显示的变化一致。本研究显示OLP的基因表达谱与健康对照有很大的不同。这些结果提供了病变生理病理过程的全局视图，这将为了解OLP的发病机制和确定新的治疗靶点提供重要线索。
The molecular biological properties of oral lichen planus (OLP) are largely unknown. The aim of this study was to identify the genes responsible for its pathogenesis at the genome scale using DNA microarray technology.The RNA samples extracted from the specimens of nine OLP patients and nine controls were analyzed with Affymetrix GeneChip. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis was applied to confirm GeneChip results.A total of 985 differentially expressed genes (629 up-regulated and 356 down-regulated) were identified. These genes were involved in many function classifications and biochemical pathways. The results of quantitative RT-PCR analysis of FOXP3, VEGFA, ANGPT1, MMP1, and SCGB2A2 were consistent with their changes demonstrated by GeneChip.This study showed the gene expression profiles of OLP, which were quite distinct from that of healthy controls. These results presented a global view of physiopathologic processes in lesions, which will give important clues to understand pathogenesis and identify new therapeutic targets of OLP.