Multifunctional chitin-based barrier membrane with antibacterial and osteogenic activities for the treatment of periodontal disease
Multifunctional chitin-based barrier membrane with antibacterial and osteogenic activities for the treatment of periodontal disease
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具有抗菌和成骨活性的多功能甲壳素基屏障膜用于治疗牙周病
DOI：
10.1016/j.carbpol.2021.118276
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发表时间：
2021
影响因子：
11.2
通讯作者：
Shi Bin
中科院分区：
文献类型：
--
作者：
Wu Tao;Huang Lin;Sun Jing;Sun Jiahui;Yan Qi;Duan Bo;Zhang Lina;Shi Bin
引导组织再生技术是修复牙周组织缺损的有效方法。然而，临床上使用的胶原屏障膜容易失去稳定性，导致软组织侵入、手术部位感染和成骨失败。一个理想的屏障膜应具有适当的抗菌，骨传导活性，和良好的生物降解。本研究首次采用碱/尿素一步溶解再生法将氧化锌纳米粒子均匀地嵌入到甲壳素水凝胶（ChT-1%ZnO）中。在150 μg/mL的溶菌酶溶液中浸泡5周后，ChT-1%ZnO的残留量为52%。ChT-1%ZnO对P. gingivalisandS.金黄色葡萄球菌6 h、12 h和24 h。此外，ChT-1%ZnO在体外具有促进成骨的作用，并在大鼠牙周缺损模型上进行了进一步的体内评价。术后8周，ChT-1%ZnO组牙釉质-牙骨质交界处厚度为1.608 mm，与无膜组（1.825 mm）和ChT组（1.685 mm）相比差异有统计学意义。
The guided tissue regeneration technique is an effective approach to repair periodontal defect. However, collagen barrier membranes used clinically lose stability easily, leading to soft tissue invasion, surgical site infection, and failure of osteogenesis. An ideal barrier membrane should possess proper antibacterial, osteoconductive activities, and favorable biodegradation. In this study, zinc oxide nanoparticles were homogeneously incorporated into the chitin hydrogel (ChT-1%ZnO) through one-step dissolution and regeneration method from alkaline/urea solution the first time. The remaining weights of ChT-1%ZnO in 150 μg/mL lysozyme solution was 52% after 5 weeks soaking. ChT-1%ZnO showed statistical antibacterial activities forP. gingivalisandS. aureusat 6 h, 12 h, and 24 h. Moreover, ChT-1%ZnO exhibits osteogenesis promotionin vitro, and it was further evaluated with rat periodontal defect modelin vivo. The cemento-enamel junction value in ChT-1%ZnO group is 1.608 mm, presenting a statistical difference compared with no-membrane (1.825 mm) and ChT group (1.685 mm) after 8 weeks postoperatively.