Specialty supplement use and biologic measures of oxidative stress and DNA damage.
Specialty supplement use and biologic measures of oxidative stress and DNA damage.
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DOI：
10.1158/1055-9965.epi-13-0470
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发表时间：
2013-12
期刊：
影响因子：
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通讯作者：
White E
中科院分区：
文献类型：
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作者：
Kantor ED;Ulrich CM;Owen RW;Schmezer P;Neuhouser ML;Lampe JW;Peters U;Shen DD;Vaughan TL;White E
氧化应激和由此产生的细胞损伤已被认为在包括癌症和心血管疾病在内的几种慢性疾病的病因学中起作用。确定与氧化应激减少和由此产生的损害相关的因素可能会指导未来的疾病预防策略。在VITALTM和生活方式（VITAL）生物标志物研究中，209人生活在西雅图地区，我们检查了目前使用的几种特殊补充剂和氧化应激，DNA损伤和DNA修复能力之间的关联。使用葡萄糖胺，软骨素，鱼油，甲基磺酰甲烷（MSM），辅酶Q10（辅酶Q10），人参，银杏，和锯棕榈是通过补充库存/采访，而使用纤维补充剂是通过问卷调查确定。超过30人使用的补充剂（葡萄糖胺和软骨素）被评估为每周药丸数量的趋势（未使用，<14粒/周，14粒/周），而不太常用的补充剂被评估为使用/未使用。采用酶免疫测定法（EIA）测定尿8-异前列腺素和PGF 2 α浓度，同时采用彗星试验测定淋巴细胞DNA损伤和DNA修复能力。使用多变量调整线性回归来模拟补充剂使用与氧化应激/DNA损伤之间的关联。使用葡萄糖胺（p趋势：0.01）、软骨素（p趋势：0.003）和纤维补充剂（p：0.01）与PGF 2 α浓度降低相关，而辅酶Q10补充剂与基线DNA损伤降低相关（p：0.003）。使用某些特殊补充剂可能与减少氧化应激和DNA损伤有关。需要进一步的研究来评估特殊补充剂的使用与氧化应激和DNA损伤标志物之间的关联。
Oxidative stress and resulting cellular damage have been suggested to play a role in the etiology of several chronic diseases, including cancer and cardiovascular disease. Identifying factors associated with reduced oxidative stress and resulting damage may guide future disease-prevention strategies. In the VITamins And Lifestyle (VITAL) biomarker-study of 209 persons living in the Seattle area, we examined the association between current use of several specialty supplements and oxidative stress, DNA damage, and DNA repair capacity. Use of glucosamine, chondroitin, fish oil, methylsulfonylmethane (MSM), co-enzyme Q10 (CoQ10), ginseng, ginkgo, and saw palmetto was ascertained by a supplement inventory/interview, while use of fiber supplements was ascertained by questionnaire. Supplements used by more than 30 persons (glucosamine and chondroitin) were evaluated as the trend across number of pills/week (non-use, <14 pills/week, 14+ pills/week), while less-commonly used supplements were evaluated as use/non-use. Oxidative stress was measured by urinary 8-isoprostane and PGF2α concentrations using enzyme immunoassays (EIA), while lymphocyte DNA damage and DNA repair capacity were measured using the Comet assay. Multivariate-adjusted linear regression was used to model the associations between supplement use and oxidative stress/DNA damage. Use of glucosamine (p-trend:0.01), chondroitin (p-trend:0.003), and fiber supplements (p:0.01) was associated with reduced PGF2α concentrations, while CoQ10 supplementation was associated with reduced baseline DNA damage (p:0.003). Use of certain specialty supplements may be associated with reduced oxidative stress and DNA damage. Further research is needed to evaluate the association between specialty supplement use and markers of oxidative stress and DNA damage.