INVITRO PHENOTYPIC AND FUNCTIONAL-CHARACTERIZATION OF HUMAN PIGMENT EPITHELIAL-CELL LINES
INVITRO PHENOTYPIC AND FUNCTIONAL-CHARACTERIZATION OF HUMAN PIGMENT EPITHELIAL-CELL LINES
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DOI：
10.3109/02713688909000023
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发表时间：
1989-05-01
影响因子：
2
通讯作者：
VERLY, G
中科院分区：
文献类型：
--
作者：
DUTT, K;WALDREP, JC;VERLY, G
我们研究了人视网膜色素上皮(HRPE)细胞表面抗原的表达。原代培养的hRPE、已建立的细胞系和新鲜的色素上皮细胞均表达人类白细胞抗原ABC，但不表达人类白细胞抗原DR。然而，原代培养和已建立的细胞系都可以被伽玛射线诱导。干扰素刺激以剂量依赖方式表达人类白细胞抗原-DR。只有新鲜刷毛的hRPE细胞表达Fc，无细胞表达C3b。我们的结果表明，hRPE细胞在培养过程中发生了变化，Fc受体的缺失反映了这一点，但在刺激下，hRPE细胞仍具有自发合成HLA-ABC和合成HLA-DR的能力。
We have characterized human retinal pigment epithelium (HRPE) for the expression of cell surface antigens. Primary HRPE cultures, established cell lines, and freshly brushed pigment epithelial cells all expess HLA-ABC but not HLA-DR antigens. However, both primary cultures and established cell lines can be induced by .gamma. interferon stimulation to express HLA-DR in a dose dependent manner. Only freshly brushed HRPE cells express Fc, and no cells demonstrated the presence of C3b. Our results show that HRPE cells change in culture, as reflected by the loss of Fc receptors, but retain the ability to synthesize HLA-ABC spontaneously and HLA-DR upon stimulation.