Effects of Schwann cells and donor antigen on long-nerve allograft regeneration
Effects of Schwann cells and donor antigen on long-nerve allograft regeneration
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DOI：
10.1002/micr.20083
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发表时间：
2005-01-01
期刊：
影响因子：
2.1
通讯作者：
Mohanakumar, T
中科院分区：
文献类型：
--
作者：
Brenner, MJ;Lowe, JB;Mohanakumar, T
同种异体神经移植已成功地用于人类受试者，以恢复创伤性神经损伤后的功能，避免随后的肢体截肢。然而，由于非特异性免疫抑制相关的发病率，这种重建方法已被限制在特别严重的神经损伤的患者。希望通过开发毒性较小的抗排斥治疗来扩大此类手术的适应症。采用小型猪神经移植模型，研究术前紫外线B（UV-B）照射供体同种异体抗原门静脉输注和注射培养的主要组织相容性复合体（MHC）匹配的雪旺细胞到神经移植物中的作用。术后20周切取移植的尺神经。组织形态计量学显示注射雪旺细胞的同种异体移植物神经再生明显增强。系列混合淋巴细胞试验表明预处理后受体对供体抗原的免疫应答受到抑制，但供体同种异体抗原预处理没有额外的神经再生作用。(C)2004 Wiley-Liss，Inc.
Nerve allotransplantation has been used successfully in human subjects to restore function after traumatic nerve injury and avoid subsequent limb amputation. However, due to the morbidity associated with nonspecific immunosuppression, this reconstructive approach has been limited to patients with particularly severe nerve injuries. It would be desirable to broaden the indications for such procedures through development of less toxic antirejection therapies. A miniature swine model of nerve transplantation was used to investigate the effects of preoperative ultraviolet-B (UV-B)-irradiated donor alloantigen portal venous infusion and injection of cultured major histocompatibility complex (MHC)-matched Schwann cells into the nerve graft. The transplanted ulnar nerves were harvested at 20 weeks. Histomorphometry showed marked enhancement in nerve regeneration through allografts injected with Schwann cells. Serial mixed lymphocyte assays demonstrated suppression of the recipient immune response to the donor antigen after pretreatment, but no additional neuroregenerative effect of donor alloantigen pretreatment. (C) 2004 Wiley-Liss, Inc.