Screening for Sulfur Compounds by Molybdenum-Catalyzed Oxidation Combined with Liquid Chromatography-Mass Spectrometry
Screening for Sulfur Compounds by Molybdenum-Catalyzed Oxidation Combined with Liquid Chromatography-Mass Spectrometry
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DOI：
10.3390/molecules25020240
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发表时间：
2020-01
期刊：
影响因子：
4.6
通讯作者：
Hirotaka Matsuo;Y. Hanamure;Rei Miyano;Yōko Takahashi;S. Ōmura;T. Nakashima
中科院分区：
文献类型：
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作者：
Hirotaka Matsuo;Y. Hanamure;Rei Miyano;Yōko Takahashi;S. Ōmura;T. Nakashima
在中性条件下，硫化物的钼（Mo）催化氧化产生砜。该反应比烯烃环氧化和伯醇或仲醇氧化更平稳地进行。本研究利用液相色谱-质谱联用技术（LC/MS），采用钼催化氧化法对微生物发酵液中的含硫化合物进行了筛选（称为“钼筛选”）。为了证明概念验证，从非硫微生物化合物的混合物中成功鉴定出已知的硫微生物化合物作为Mo催化氧化的亚磺酰基或磺酰基产物。然后，我们的二硫化钼筛选方法被用来筛选300个样品的微生物培养液的硫化合物。其中一种已鉴定的化合物是由放线菌菌株产生的含有N-乙酰半胱氨酸部分的吉他西他林。这些结果证明了在从微生物来源中寻找新的硫化合物时，MoS筛选的潜力。
The molybdenum (Mo)-catalyzed oxidation of sulfide under neutral conditions yields sulfone. This reaction proceeds more smoothly than olefin epoxidation and primary or secondary alcohol oxidation. In this study, Mo-catalyzed oxidation was used to screen for sulfur compounds (named “MoS-screening”) in microbial broths by liquid chromatography-mass spectrometry (LC/MS). To demonstrate proof-of-concept, known sulfur microbial compounds were successfully identified from a mixture of non-sulfur microbial compounds as sulfinyl or sulfonyl products of Mo-catalyzed oxidation. Then our MoS-screening method was used to screen 300 samples of microbial broth for sulfur compounds. One of the identified compounds was a kitasetaline-containing N-acetyl cysteine moiety produced by an actinomycete strain. These results demonstrate the potential of MoS-screening in the search for new sulfur compounds from microbial sources.