ANKYRIN(G) - A NEW ANKYRIN GENE WITH NEURAL-SPECIFIC ISOFORMS LOCALIZED AT THE AXONAL INITIAL SEGMENT AND NODE OF RANVIER
ANKYRIN(G) - A NEW ANKYRIN GENE WITH NEURAL-SPECIFIC ISOFORMS LOCALIZED AT THE AXONAL INITIAL SEGMENT AND NODE OF RANVIER
复制标题
DOI：
10.1074/jbc.270.5.2352
复制
发表时间：
1995-02-03
影响因子：
4.8
通讯作者：
BENNETT, V
中科院分区：
文献类型：
--
作者：
KORDELI, E;LAMBERT, S;BENNETT, V
我们已经鉴定了一个新的在大脑和其他组织中表达的Ankyrin基因，它经历了广泛的组织特异性替代mRNA加工，全长多肽的分子质量为480 kDa，包括一个预测的球状头部结构域，具有膜和血影蛋白结合活性，以及一个延伸的‘尾’结构域，我们将该基因命名为ankyrin(G)，基于其巨大的大小和普遍表达，我们鉴定了两个大脑特异的亚型，480 kDa和270 kDa，含有紧随球形头部结构域的高度富含丝氨酸和苏氨酸残基的独特序列，抗丝氨酸结构域和血影蛋白结合域的抗体显示了Ranvier和轴突起始节段的结节的标记，Ankyrin结合蛋白也分布在这些特化的膜域中，包括电压依赖的钠通道、钠/钾ATPase、钠/钙交换器和神经束素/L1家族的细胞黏附分子，神经特异性的ankyrin(G)多肽是参与维持/靶向Ranvier和轴突起始节段的离子通道和细胞黏附分子的候选分子。
We have characterized a new ankyrin gene, expressed in brain and other tissues, that is subject to extensive tissue-specific alternative mRNA processing, The full-length polypeptide has a molecular mass of 480 kDa and includes a predicted globular head domain, with membrane- and spectrin-binding activities, as well as an extended ''tail'' domain, We term this gene ankyrin(G) based on its giant size and general expression, Two brain-specific isoforms of 480 kDa and 270 kDa were identified that contain a unique stretch of sequence highly enriched in serine and threonine residues immediately following the globular head domain, Antibodies against the serine-rich domain and spectrin-binding domain revealed labeling of nodes of Ranvier and axonal initial segments, Ankyrin-binding proteins also known to be localized in these specialized membrane domains include the voltage-dependent sodium channel, the sodium/potassium ATPase, sodium/calcium exchanger, and members of the neurofascin/L1 family of cell adhesion molecules, The neural-specific ankyrin(G) polypeptides are candidates to participate in maintenance/targeting of ion channels and cell adhesion molecules to nodes of Ranvier and axonal initial segments.