Type 2 immunity-dependent reduction of segmented filamentous bacteria in mice infected with the helminthic parasite Nippostrongylus brasiliensis.
Type 2 immunity-dependent reduction of segmented filamentous bacteria in mice infected with the helminthic parasite Nippostrongylus brasiliensis.
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DOI：
10.1186/s40168-015-0103-8
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发表时间：
2015-09-17
期刊：
影响因子：
15.5
通讯作者：
Zhao A
中科院分区：
文献类型：
--
作者：
Fricke WF;Song Y;Wang AJ;Smith A;Grinchuk V;Mongodin E;Pei C;Ma B;Lu N;Urban JF Jr;Shea-Donohue T;Zhao A
宿主和胃肠道微生物群之间的动态相互作用对局部和全身免疫稳态起着重要作用。蠕虫寄生虫调节宿主的免疫反应，导致对自身免疫性疾病的保护，但也增加了对病原体感染的易感性。其潜在机制在很大程度上仍然未知。我们发现，2型免疫反应肠道日本圆线虫感染的小鼠与改变肠粘蛋白和AMP的表达和微生物群组成的变化。最引人注目的是，感染降低了肠道分节丝状菌（SFB）的浓度，已知的辅助性T细胞17的诱导剂，和IL-17相关基因的表达。IL-13或STAT 6缺陷的感染小鼠不降低SFB或IL-17，外源性IL-25复制了野生型小鼠中寄生虫感染的效果。我们的数据表明，寄生虫感染通过宿主2型免疫来减少肠道SFB和IL-17的表达，提供了寄生虫依赖于微生物的免疫调节的例子。本文的在线版本（doi：10.1186/s40168-015-0103-8）包含补充材料，可供授权用户使用。
Dynamic interactions between the host and gastrointestinal microbiota play an important role for local and systemic immune homeostasis. Helminthic parasites modulate the host immune response, resulting in protection against autoimmune disease but also increased susceptibility to pathogen infection. The underlying mechanisms remain largely unknown. We showed that the type 2 immune response to enteric Nippostrongylus brasiliensis infection in mice was associated with altered intestinal mucin and AMP expression and shifts in microbiota composition. Most strikingly, infection reduced concentrations of intestinal segmented filamentous bacteria (SFB), known inducers of T helper 17 cells, and IL-17-associated gene expression. Infected mice deficient in IL-13 or STAT6 did not reduce SFB or IL-17, and exogenous IL-25 replicated the effects of parasite infection in wild type mice. Our data show that parasite infection acts through host type 2 immunity to reduce intestinal SFB and expression of IL-17, providing an example of a microbiota-dependent immune modulation by parasites. The online version of this article (doi:10.1186/s40168-015-0103-8) contains supplementary material, which is available to authorized users.