Effects of lateral preoptic area application of orexin-A on sleep-wakefulness.
Effects of lateral preoptic area application of orexin-A on sleep-wakefulness.
复制标题
外侧视前区应用食欲素-A 对睡眠-觉醒的影响。
DOI：
10.1097/00001756-200011090-00004
复制
发表时间：
2000
期刊：
影响因子：
1.7
通讯作者：
McGinty,D
中科院分区：
文献类型：
--
作者：
Methippara,MM;Alam,MN;Szymusiak,R;McGinty,D
食欲素是一种新发现的下丘脑肽，缺乏食欲素被认为会导致人类嗜睡症和动物嗜睡症模型中的异常嗜睡和猝倒。由于POA含有广泛的食欲素末端，并被确定为睡眠/觉醒调节位点，我们评估了一个假设，即该位点是食欲素唤醒诱导作用的靶点。将Orexin-A微注射于外侧视前区（IPOA），观察其对睡眠-觉醒和脑温度的影响。与生理盐水对照相比，食欲素a诱导醒觉增加70 min，抑制所有睡眠阶段，特别是SWS2和REM分别持续80和90 min。与生理盐水对照组相比，食欲素a对脑温度的影响没有差异。食欲素诱导的觉醒和REM抑制与嗜睡症的食欲素缺乏模型一致。我们的研究结果表明，IPOA食欲素末端区或邻近结构可能是该肽觉醒调节的一个位点，也是发作性睡症睡眠-觉醒调节缺陷的一个底物。
Deficiency of orexin, a newly discovered hypothalamic peptide, is thought to lead to abnormal sleepiness and cataplexy in both human narcolepsy and animal models of the disease. As the POA contains extensive orexin terminals and is established as a sleep/arousal regulatory site, we evaluated a hypothesis that this site is a target for the arousal-inducing effects of orexin. Orexin-A was microinjected into lateral preoptic area (IPOA) and the effects on sleep–wakefulness and brain temperature were studied. Compared to saline vehicle control, orexin-A induced an increase in wakefulness for 70 min and suppressed all sleep stages, especially SWS2 and REM for 80 and 90 min, respectively. Brain temperature was not differentially affected by orexin-A compared to saline control. The orexin-induced arousal and REM suppression are consistent with the orexin-deficiency model of narcolepsy. Our results suggest that the IPOA orexin terminal field or adjacent structures may be a locus of arousal regulation by this peptide and a substrate of sleep-wake regulatory deficits in narcolepsy.