Neutralization of HPV16, 18, 31, and 58 pseudovirions with antisera induced by immunizing rabbits with synthetic peptides representing segments of the HPV 16 minor capsid protein L2 surface region
Neutralization of HPV16, 18, 31, and 58 pseudovirions with antisera induced by immunizing rabbits with synthetic peptides representing segments of the HPV 16 minor capsid protein L2 surface region
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DOI：
10.1016/j.virol.2006.08.037
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发表时间：
2007-02-20
期刊：
影响因子：
3.7
通讯作者：
Kanda, Tadahito
中科院分区：
文献类型：
--
作者：
Kondo, Kazunari;Ishii, Yoshiyuki;Kanda, Tadahito
抗人乳头瘤病毒（HPV）小衣壳蛋白L2中和抗体在体外可交叉中和不同基因型的HPV。为了鉴定含有HPV 16 L2的交叉中和表位的片段，我们表征了通过用14至20个氨基酸（aa）长的10种合成肽中的每一种免疫两只兔子获得的抗血清，所述合成肽代表HPV 16 L2序列的一部分，从14至144。抗AS 18 - 144区域内的肽的抗血清有效地结合HPV 16 L1/L2-衣壳并中和HPV 16假病毒体，表明该区域展示在衣壳表面并含有几个中和表位。抗as 18至38肽的抗血清（抗P18/38）交叉中和HPV 18。抗P56/75交叉中和HPV 18、31和58。抗P61/75和抗P64/81交叉中和HPV 18和58。抗P96/115和突变型P96/115诱导的抗血清（101和112处的S和T分别替换为L和S）交叉中和HPV 31和58。等体积的抗P18/38、抗P56/75和抗突变型P96/115三种抗血清的混合物比单独的抗P56/75更有效地中和HPV 16、18、31和58，表明抗体对交叉中和有协同作用。交叉中和似乎与HPV类型之间的保守序列相关。本研究中的数据为设计有效对抗更广谱的高危HPV的疫苗抗原提供了基础。(c)2006年爱思唯尔公司All rights reserved.
Neutralizing antibody against human papillomavirus (HPV) minor capsid protein L2 can cross-neutralize different HPV genotypes in vitro. To identify the segments containing the cross-neutralization epitopes of HPV 16 L2, we characterized antisera obtained by immunizing two rabbits with each of the ten synthetic peptides of 14 to 20 amino acids (aa) long, which represents a part of the HPV 16 L2 sequence from as 14 to 144. The antisera against the peptides within the region from as 18 to 144 efficiently bound to HPV 16 L1/L2-capsids and neutralized HPV 16 pseudovirions, indicating that the region is displayed on the surface of the capsids and contains several neutralization epitopes. Antiserum against the peptide from as 18 to 38 (anti-P18/38) cross-neutralized HPV 18. Anti-P56/75 cross-neutralized HPV18, 31, and 58. Anti-P61/75 and anti-P64/81 cross-neutralized HPV 18 and 58. Anti-P96/115 and the antiserum induced by a mutant P96/115 (S and T at as 101 and 112 were replaced with L and S, respectively) cross-neutralized HPV31 and 58. The mixture of equal volumes of three antisera, anti-P18/38, anti-P56/75, and anti-mutant P96/115, neutralized HPV 16, 18, 31, and 58 more efficiently than anti-P56/75 alone, suggesting that there is a synergistic effect of antibodies on the cross-neutralization. The cross-neutralization appears to be correlated with conserved as sequences among HPV types. The data in this study provide a basis for designing vaccine antigens effective against a broader spectrum of the high-risk HPVs. (c) 2006 Elsevier Inc. All rights reserved.