Heterogeneity of chromosome 22 breakpoint in Philadelphia-positive (Ph+) acute lymphocytic leukemia.
Heterogeneity of chromosome 22 breakpoint in Philadelphia-positive (Ph+) acute lymphocytic leukemia.
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DOI：
10.1073/pnas.83.6.1807
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发表时间：
1986-03
影响因子：
11.1
通讯作者：
J. Erikson;Constance A. GRIFFINt;A. Ar-rushdi;M. Valtieri;James Hoxiem;Janet FINANt;Beverly S. EMANUELt-Beverly-S
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文献类型：
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作者：
J. Erikson;Constance A. GRIFFINt;A. Ar-rushdi;M. Valtieri;James Hoxiem;Janet FINANt;Beverly S. EMANUELt-Beverly-S
在t(9;22)（q34;q11）染色体易位的慢性髓性白血病（CML）中，22号染色体上的断点发生在5.8千碱基的DNA片段内，称为“断点簇区”（bcr）。大约10%的急性淋巴细胞白血病（ALL）患者发生相同的细胞遗传学上难以区分的易位。在这项研究中，我们研究了几例携带t（9;22）易位的ALL患者的染色体断点。在5例ALL病例中的3例中，我们发现bcr区不参与染色体重排，22q11染色体断点在22q11带的bcr区近端（5'）。此外，我们在携带t（9;22）易位的ALL细胞系中观察到正常大小的bcr和c-abl转录本。因此，我们得出结论，如果c-abl在没有bcr重排的ALL细胞中不适当表达，那么激活的遗传机制一定与CML中报道的不同。
In chronic myelogenous leukemias (CML) with the t(9;22)(q34;q11) chromosome translocation the breakpoints on chromosome 22 occur within a 5.8-kilobase segment of DNA referred to as "breakpoint cluster region" (bcr). The same cytogenetically indistinguishable translocation occurs in approximately 10% of patients with acute lymphocytic leukemias (ALL). In this study we have investigated the chromosome breakpoints in several cases of ALL carrying the t(9;22) translocation. In three of five cases of ALL we found that the bcr region was not involved in the chromosome rearrangement and that the 22q11 chromosome breakpoints were proximal (5') to the bcr region at band 22q11. In addition, we observed normal size bcr and c-abl transcripts in an ALL cell line carrying the t(9;22) translocation. We conclude, therefore, that if c-abl is inappropriately expressed in ALL cells without bcr rearrangements, the genetic mechanism of activation must be different from that reported for CML.