Biochemical pathways and associated microbial process of di-2-ethyl hexyl phthalate (DEHP) enhanced degradation by the immobilization technique in sequencing batch reactor
Biochemical pathways and associated microbial process of di-2-ethyl hexyl phthalate (DEHP) enhanced degradation by the immobilization technique in sequencing batch reactor
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序批式反应器中固定化技术增强邻苯二甲酸二-2-乙基己酯（DEHP）降解的生化途径和相关微生物过程
DOI：
10.1080/09593330.2021.1909657
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发表时间：
2021-03
影响因子：
2.8
通讯作者：
Lin Li
中科院分区：
文献类型：
--
作者：
Jia Chen;Siqiao Yang;Ke Zhang;Wei Chen;You Mo;Lin Li
摘要从活性污泥中成功分离到一株细菌ASLT-13，经鉴定为杏仁假单胞菌。气相色谱-质谱仪(GC-MS)分析表明，菌株ASLT-13能完全矿化邻苯二甲酸二异辛酯(DEHP)。在降解基因的作用下，DEHP首先从苯环的较长侧链代谢成邻苯二甲酸二丁酯等较短的分支(邻苯二甲酸单酯)。然后将DBP转化为邻苯二甲酸二甲酯(DMP)，然后将其水解为邻苯二甲酸(PA)。最终，PA通过TCA循环转化为CO2和H2O。最佳固定化条件为：海藻酸钠(SA)浓度6%，CaCl2浓度5%，菌液比1：1，交联时间6 h。结果表明，固定化显著提高了DEHP的去除效率。节杆菌属、不动杆菌属、芽孢杆菌属和红球菌属是降解DEHP的优势属。本研究表明，细胞固定化技术在DEHP废水处理中具有潜在的应用前景。图形摘要
ABSTRACT A bacterial strain ASLT-13 was successfully isolated from activated sludge and identified as Pseudomonas amygdali. Gas chromatograph-mass spectrometer (GC-MS) analysis indicated that strain ASLT-13 could completely mineralize di 2-ethyl hexyl phthalate (DEHP). DEHP was first metabolized from the longer side chain of the benzene ring into shorter branches (Phatlalic mono-esters) like Dibutyl phthalate (DBP) under the action of degrading genes. DBP was then converted into di-methyl phthalate (DMP), and then hydrolysed to phthalic acid (PA). PA was eventually converted to CO2 and H2O through the TCA cycle. The optimal conditions for immobilization were the sodium alginate (SA) concentration of 6%, CaCl2 concentration of 5%, ratio of bacteria and SA of 1:1, crosslinking time of 6 h. Bacterial quantity and community structure in sequencing batch reactors (SBRs) was investigated by q-PCR and high-throughput sequencing. The results indicated that DEHP removal efficiency was significantly enhanced by immobilization. Arthrobacter, Acinetobacter, Bacillus and Rhodococcus were the predominant genera for DEHP degradation. This study suggested that the cell immobilization technology had a potential application in DEHP wastewater treatment. GRAPHICAL ABSTRACT
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影响因子：
4.4
作者：
Idris, A;Suzana, W
通讯作者：
Suzana, W
影响因子：
8.9
作者：
Ha, Mei;Wei, Li;Liu, Changjiang
通讯作者：
Liu, Changjiang
DOI：
10.1023/a:1020108502776
发表时间：
2002-10
期刊：
Water, Air, and Soil Pollution
影响因子：
--
作者：
Zeng Feng;Cui Kunyan;Fu Jiamo;S. Guoying;Yang Huifang
通讯作者：
Zeng Feng;Cui Kunyan;Fu Jiamo;S. Guoying;Yang Huifang
影响因子：
6.1
作者：
Ke Zhang;Yihao Liu;Hongbing Luo;Qiang Chen;Zhanyuan Zhu;Wei Chen-;Jia Chen;Lin Ji;You Mo
通讯作者：
Ke Zhang;Yihao Liu;Hongbing Luo;Qiang Chen;Zhanyuan Zhu;Wei Chen-;Jia Chen;Lin Ji;You Mo
DOI：
10.1016/j.ibiod.2018.04.006
发表时间：
2018-08-01
影响因子：
4.8
作者：
Zhang, Jingfan;Zhang, Chengnan;Li, Xiuting
通讯作者：
Li, Xiuting