Root-Specific DNA Methylation in Chloris virgata, a Natural Alkaline-Resistant Halophyte, in Response to Salt and Alkaline Stresses
Root-Specific DNA Methylation in Chloris virgata, a Natural Alkaline-Resistant Halophyte, in Response to Salt and Alkaline Stresses
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虎尾草（一种天然耐碱盐生植物）响应盐和碱胁迫的根部特异性 DNA 甲基化
DOI：
10.1007/s11105-012-0420-z
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发表时间：
2012-10-01
影响因子：
2.1
通讯作者：
Shi, Decheng
中科院分区：
文献类型：
--
作者：
Cao, Donghui;Gao, Xiang;Shi, Decheng
DNA甲基化一直被认为在植物对多种环境胁迫的反应中起着重要的调节作用。与盐渍土一样，碱性土壤是重要的农业污染物，对植物代谢，特别是根系生理有复杂的影响。然而，目前还没有关于植物对盐碱胁迫的表观遗传反应的报道。在这项研究中，我们报告的盐胁迫（S，1：1摩尔比的NaCl到Na 2SO 4）以及碱胁迫（A，1：1摩尔比的NaHCO 3到Na 2CO 3）对DNA甲基化的影响在一个耐碱性盐生植物，虎尾草。利用21对甲基化敏感扩增多态性引物，在叶和根中分别扩增出959和1,040条DNA片段。结果表明，叶中甲基化水平为18.35%，根中甲基化水平为12.40%。此外，盐和碱胁迫引起DNA甲基化的变化，主要是在根。盐胁迫下叶片变异率为0.75%，根系变异率为5.29%;碱胁迫下叶片变异率为1.26%，根系变异率为14.17%。结果表明，碱胁迫比盐胁迫更具破坏性和复杂性，根中DNA甲基化调控可能在盐碱胁迫抗性的获得和遗传中起重要作用。
DNA methylation has long been considered to play important roles in the regulation of plant response to multiple environmental stresses. As it is with saline soils, alkaline soils are important agricultural contaminants that have complex effects on plant metabolism and, in particular, on root physiology. However, there are no reports on epigenetic responses of plants to both salt and alkaline stresses. In this study, we report on the effects of salt stress (S, 1:1 molar ratio of NaCl to Na2SO4) as well as alkaline stress (A, 1:1 molar ratio of NaHCO3 to Na2CO3) on DNA methylation in an alkaline-resistant halophyte, Chloris virgate. A total of 959 and 1,040 DNA fragments were amplified in leaves and roots, respectively, by using 21 pairs of selective primers in methylation-sensitive amplified polymorphism analysis. The results showed that the overall level of methylation in leaves was 18.35%, and in roots, it was 12.40%. Furthermore, both salt and alkaline stress caused DNA methylation variations, predominantly in the roots. The variation ratio was 0.75% in leaves and 5.29% in roots under salt stress and 1.26% in leaves and 14.17% in roots under alkaline stress. The results suggested that alkaline stress was more destructive and complex than salt stress and that the DNA methylation regulation that occurred in the roots might play an important role in the acquirement and inheritance of salt and alkaline stress tolerance.