Epstein–Barr virus is present in neoplastic cytotoxic T cells in extranodal, and predominantly in B cells in nodal T non‐Hodgkin lymphomas
Epstein–Barr virus is present in neoplastic cytotoxic T cells in extranodal, and predominantly in B cells in nodal T non‐Hodgkin lymphomas
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Epstein-Barr 病毒存在于结外肿瘤细胞毒性 T 细胞中，主要存在于结节 T 非霍奇金淋巴瘤的 B 细胞中
作者：
A. Brink;R. L. ten Berge;A. V. D. van den Brule;R. Willemze;A. Chott;C. Meijer
已经描述了EB病毒（EBV）阳性T非霍奇金淋巴瘤（T-NHL），但目前尚不清楚EBV如何感染T淋巴细胞。据推测，细胞毒性T细胞（CTL）或自然杀伤（NK）细胞可以在杀死EBV感染的靶细胞期间被EBV感染。因此，本研究的目的是确定不同位置的EBV阳性T-NHL（n=221）中的肿瘤细胞是否具有细胞毒性表型。为了鉴定携带EBV的细胞，结合使用EBV编码RNA（EBER）原位杂交（RISH）和免疫组织化学法检测T和B细胞标志物以及细胞毒性蛋白TIA-1和颗粒酶B。在所有鼻T-NHL（n=9）、5/34例胃肠道（GI）T-NHL和2/6例肺T-NHL的肿瘤细胞中检测到EBV，但在原发性皮肤T-NHL（n=103）中未检测到EBV。此外，在2/48个淋巴结间变性大细胞淋巴瘤（ALCL）的肿瘤细胞中发现了EBV，但在其他淋巴结T-NHL的肿瘤T细胞中未发现EBV。然而，5/17例未另行说明的淋巴结外周T-NHL（PTCL NOS）和1/4例T-幼淋巴细胞白血病确实含有EBV阳性非T细胞。双重染色显示，在EBV阳性淋巴结T-NHL（n=16）中，EBER阳性细胞具有细胞毒性表型（TIA-1和/或颗粒酶B-阳性）。在淋巴结非ALCL T-NHL中，EBER阳性细胞的TIA-1或颗粒酶B不呈阳性，也不表达CD 3、CD 21或HECA 452。相反，这些细胞中的大多数表达B-细胞标志物CD 20。这些具有EBER阳性细胞的PTCL NOS显示出AILD样T-NHL的特征。结论是，EBV阳性结节性T-NHL的肿瘤细胞总是具有细胞毒性表型，支持EBV可以感染CTL的观点。在淋巴结非ALCL T-NHL中，EBV仅见于具有AILD样特征的T-NHL中，并存在于B细胞中，可能导致恶性B-细胞克隆的生长。版权所有© 2000约翰威利父子有限公司。
Epstein–Barr virus (EBV)‐positive T non‐Hodgkin lymphomas (T‐NHLs) have been described, but it is at present unknown how EBV infects T lymphocytes. It has been postulated that cytotoxic T cells (CTLs) or natural killer (NK) cells can be infected by EBV during the killing of an EBV‐infected target cell. The objective of this study was therefore to determine whether the neoplastic cells in EBV‐positive T‐NHLs (n=221) of various locations have a cytotoxic phenotype. To identify EBV‐harbouring cells, combinations were used of EBV‐encoded RNA (EBER) in situ hybridization (RISH) and immunohistochemistry for T‐ and B‐cell markers and the cytotoxic proteins TIA‐1 and granzyme B. EBV was detected in the neoplastic cells of all nasal T‐NHLs (n=9), 5/34 gastrointestinal (GI) T‐NHLs, and 2/6 lung T‐NHLs, but not in primary cutaneous T‐NHLs (n=103). Moreover, EBV was found in the neoplastic cells of 2/48 nodal anaplastic large cell lymphomas (ALCLs), but not in neoplastic T cells of other nodal T‐NHLs. However, 5/17 nodal peripheral T‐NHLs not otherwise specified (PTCLs NOS) and 1/4 T‐prolymphocytic leukaemias did contain EBV‐positive non‐T cells. Double staining revealed that in EBV‐positive extranodal T‐NHLs (n=16), the EBER‐positive cells had a cytotoxic phenotype (TIA‐1‐ and/or granzyme B‐positive). In nodal non‐ALCL T‐NHLs, the EBER‐positive cells were not positive for TIA‐1 or granzyme B, nor did they express CD3, CD21 or HECA452. Instead, most of these cells expressed the B‐cell marker CD20. These PTCLs NOS with EBER‐positive cells showed features of AILD‐like T‐NHL. It is concluded that neoplastic cells of EBV‐positive extranodal T‐NHLs always have a cytotoxic phenotype, supporting the view that EBV can infect CTLs. In nodal non‐ALCL T‐NHL, EBV is only found in T‐NHL with AILD‐like features and is present in B cells, where it may contribute to the outgrowth of a malignant B‐cell clone. Copyright © 2000 John Wiley & Sons, Ltd.
单克隆抗体 HECA-452 定义了人淋巴组织和炎症组织中的高内皮分化。
DOI：
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发表时间：
1988
期刊：
The American journal of pathology
影响因子：
--
作者：
Duijvestijn,AM;Horst,E;Pals,ST;Rouse,BN;Steere,AC;Picker,LJ;Meijer,CJ;Butcher,EC
通讯作者：
Butcher,EC
颗粒酶 B 簇蛋白酶在细胞介导的细胞毒性中的作用。
DOI：
10.1006/smim.1997.0060
发表时间：
1997
期刊：
Seminars in immunology.
影响因子：
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作者：
Pham,CT;Ley,TJ
通讯作者：
Ley,TJ
SCID/hu 小鼠 Epstein-Barr 病毒诱导的 B 细胞肿瘤中 CD20 和 CD23 低表达。
DOI：
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发表时间：
1993
期刊：
The American journal of pathology
影响因子：
--
作者：
Garnier,JL;Cooper,NR;Cannon,MJ
通讯作者：
Cannon,MJ