SERUM FERRITIN IN PATIENTS WITH CANCER - DETERMINATION WITH ANTIBODIES TO HELA-CELL AND SPLEEN FERRITIN
SERUM FERRITIN IN PATIENTS WITH CANCER - DETERMINATION WITH ANTIBODIES TO HELA-CELL AND SPLEEN FERRITIN
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DOI：
10.1016/0009-8981(80)90173-4
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发表时间：
1980-01-01
影响因子：
5
通讯作者：
JACOBS, A
中科院分区：
文献类型：
--
作者：
JONES, BM;WORWOOD, M;JACOBS, A
一些恶性组织和细胞系含有酸性异铁蛋白；血清中这种异铁蛋白的测定可能对恶性肿瘤的诊断有价值。本文描述了从人宫颈癌细胞中纯化的酸性铁蛋白的放射免疫测定。纯化的心脏、肾脏、肝脏和脾脏铁蛋白检测表明，该方法对酸性异铁蛋白具有高度特异性。用HeLa细胞抗体和脾铁蛋白抗体测定正常组织和肿瘤组织提取物中的铁蛋白浓度。虽然肿瘤中HeLa型铁蛋白含量高于相应的正常组织，但HeLa/脾型铁蛋白比例较低。将hela型铁蛋白与抗脾铁蛋白在< 1000例正常人、癌症和白血病患者血清中的含量进行比较。未检出hela型铁蛋白（< 2 .mu）。G /l)。浓度高达53 .亩。恶性肿瘤患者血清中铁蛋白含量均为g/l，但HeLa/脾型铁蛋白比例一直很低。HeLa细胞抗体或心脏铁蛋白抗体在癌症的诊断或监测中似乎很少应用。
Some malignant tissues and cell lines contain acidic isoferritins; the assay of such isoferritins in serum may be of value in the diagnosis of malignancy. A radioimmunoassay for acidic ferritin purified from HeLa [human cervical carcinoma] cells is described. Examination of purified heart, kidney, liver and spleen ferritin showed that the assay was highly specific for acidic isoferritins. Ferritin concentrations were measured with antibodies to HeLa cell and spleen ferritin in extracts of normal and tumor tissue. Although the tumors contained more HeLa type ferritin than the corresponding normal tissue the HeLa/spleen type ferritin ratio was low. HeLa-type ferritin concentrations were compared with values obtained with anti-spleen ferritin in < 1000 sera from normal subjects and patients with cancer and leukemia. HeLa-type ferritin was not detected (< 2 .mu.g/l) in most normal sera. Concentrations of up to 53 .mu.g/l were found in sera from patients with malignant disease but the HeLa/spleen type ferritin ratio was always very low. There appears to be little application for antibodies to HeLa cell or heart ferritin in the diagnosis or monitoring of cancer.